pGFP Vector
Green fluorescent protein (GFP) is a naturally fluorescent protein from Aequorea victoria that can be used as an in vivo reporter of gene expression and protein localization. pGFP is a convenient source of the GFP cDNA. The GFP coding sequence is flanked by separate MCSs at the 5' and 3' ends, so the GFP gene can be easily be excised. Alternatively, the GFP coding sequences can be amplified by PCR.
Overview
- Green fluorescent proteins from Aequorea victoria
- pGFP encodes wild-type GFP
- pGFPuv encodes a GFP variant optimized for maximal fluorescence when excited by standard UV light (360–400 nm)
Applications
- Bacterial expression
- Sources for the GFP and GFPuv fluorescent protein coding sequences
pGFP and pGFP-UV vector maps
pGFP and pGFP-UV vector maps. Note: There is a stop codon at the 5' end of the 3' MCS. The 3' MCS should not be used for cloning.
Crameri, A., Whitehorn, E. A., Tate, E. & Stemmer, W. P. Improved green fluorescent protein by molecular evolution using DNA shuffling. Nat. Biotechnol. 14, 315-9 (1996).
Write Your Own Review
pGFP and pGFP-UV vector maps
pGFP and pGFP-UV vector maps. Note: There is a stop codon at the 5' end of the 3' MCS. The 3' MCS should not be used for cloning.
