Pfu Pyroglutamate Aminopeptidase liberates the N-terminal pyroglutamic acid from proteins and peptides. This enzyme may work well with some intact, non-denatured protein. In such cases, a denaturation step may be unnecessary. This product is supplied with 5X Reaction Buffer [250 mM sodium phosphate (pH 7.0), 50 mM DTT, 5 mM EDTA].

Applications

• Removal of pyroglutamic acids from the N-termini of proteins and peptides

Components

    Supplied Buffer

• Volume: 1 ml
• Component: 250 mM sodium phosphate buffer (pH 7.0) containing 50 mM DTT and 5 mM EDTA
• Comparison of specific activities across different temperatures: 5.83 U/mg protein at 37°C showed activities of 12.2 U/mg protein or 28.3 U/mg protein at 50°C or 75°C, respectively.

Source

Recombinant Escherichia coli encoding the Pyrococcus furiosus pyroglutamate aminopeptidase gene.

Purity

Greater than or equal to 90% via SDS-PAGE. No other protease activity detected.