Pfu Protease S is an endo-type serine protease with broad activity toward native and denatured proteins. Cleavage occurs primarily on the carboxy side of peptide bonds of hydrophobic amino acid residues.
Applications
Digestion of proteins and peptides for primary structure analysis
Source
Recombinant Bacillus encoding the Pyrococcus furiosus protease gene
Purity
Homogeneous via SDS-PAGE. No other proteases detected.
1% SDS; retains 50% activity after 24 hr at 95°C, pH 7.0
6.4 M urea; retains 70% activity after 1 hr at 95°C, pH 7.0
50% acetonitrile; retains 90% activity after 1 hr at 95°C, pH 7.0
Definition of activity
One unit of activity corresponds to the amount of enzyme required to hydrolyze 1 mmol of N-succinyl Ala-Ala-Pro-Phe p-nitroanilide in 1 minute at 95°C and pH 7.0.
Form
In a solution of 25 mM Tris-HCl (pH 7.6) containing 40% ethanol
Pfu Protease S is an endo-type serine protease with broad activity toward native and denatured proteins. Cleavage occurs primarily on the carboxy side of peptide bonds of hydrophobic amino acid residues.
Applications
Digestion of proteins and peptides for primary structure analysis
Source
Recombinant Bacillus encoding the Pyrococcus furiosus protease gene
Purity
Homogeneous via SDS-PAGE. No other proteases detected.
1% SDS; retains 50% activity after 24 hr at 95°C, pH 7.0
6.4 M urea; retains 70% activity after 1 hr at 95°C, pH 7.0
50% acetonitrile; retains 90% activity after 1 hr at 95°C, pH 7.0
Definition of activity
One unit of activity corresponds to the amount of enzyme required to hydrolyze 1 mmol of N-succinyl Ala-Ala-Pro-Phe p-nitroanilide in 1 minute at 95°C and pH 7.0.
Form
In a solution of 25 mM Tris-HCl (pH 7.6) containing 40% ethanol
