pEXP-Lib Vector
pEXP-Lib Vector contains an internal ribosome entry site (IRES), which permits the translation of two open reading frames from one messenger RNA. After selection with puromycin, nearly all surviving colonies will stably express the gene of interest, thus decreasing the need to screen large numbers of colonies to find functional clones. SfiIA and SfiIB indicate two distinct SfiI sites that differ in their interpalindromic sequences. This arrangement permits cloning of libraries such that all inserts are in the proper orientation for protein translation.
pEXP-Lib vector map
pEXP-Lib vector map. pEXP-Lib is a mammalian expression vector that permits the screening and analysis of libraries directly in mammalian cell lines.
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pEXP-Lib vector map
pEXP-Lib vector map. pEXP-Lib is a mammalian expression vector that permits the screening and analysis of libraries directly in mammalian cell lines.
