pET Expression Systems
pET Expression Systems
Brand: Takara Bio.
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pET Expression Systems
The pET express & purify system vectors—pET6xHN
Takara's pET express and purify kits contain pET vectors (the pET6XHN series of vectors) which encode N- or C-terminal 6xHN fusion tags. These are available in a choice of cloning formats (easy In-Fusion Cloning or traditional restriction enzyme cloning) for maximum flexibility tailored to your expression needs.
pET express & purify kits—a complete expression & purification system
The pET express & purify kits supply a choice of IMAC resins and buffers to purify expressed his-tagged proteins. You can choose His60 Ni nickel-based resin for high binding capacity or HisTALON cobalt-based resin for high purity. The kits are supplied with prepacked gravity columns filled with either resin and provide all of the buffers necessary to perform the protein extraction and purification.
Overview
Cobalt-based resin—HisTALON
- No copurification of unwanted host proteins
- No SlyD contamination (histidine-rich protein present in E. coli)
- Lowest metal ion leakage
- Available in various formats as bulk resin, cartridges, and gravity columns
Nickel-based resin—His60 Ni Superflow Resin
- 60 mg/ml binding capacity
- Up to 95% purity
- Low metal ion leakage
- Available in various formats as bulk resin, cartridges, and gravity columns
Features
- Powerful—Higher inducible protein expression levels and tighter control of your target gene
- Fast—E. coli BL21(DE3)-based system
- Versatile—Choose between N- & C-terminal 6xHN-tagged vectors
- Convenient—Choice of fast, simple In-Fusion PCR cloning or traditional T4 DNA ligase cloning
- Complete—Purify your expressed protein with His60 Nickel resin or TALON Cobalt resin
The pET Express & Purify Kit protocol
The pET Express & Purify Kit protocol.
Cloning options using the pET Express & Purify Kits
Cloning options using the pET Express & Purify Kits.
The molecular basis of recombinant protein expression in uninduced bacterial host cells using the pET system
The molecular basis of recombinant protein expression in uninduced bacterial host cells using the pET system.
The molecular basis of recombinant protein expression in IPTG-induced bacterial host cells using the pET system
The molecular basis of recombinant protein expression in IPTG-induced bacterial host cells using the pET system.
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The pET Express & Purify Kit protocol
The pET Express & Purify Kit protocol.
Cloning options using the pET Express & Purify Kits
Cloning options using the pET Express & Purify Kits.
The molecular basis of recombinant protein expression in uninduced bacterial host cells using the pET system
The molecular basis of recombinant protein expression in uninduced bacterial host cells using the pET system.
The molecular basis of recombinant protein expression in IPTG-induced bacterial host cells using the pET system
The molecular basis of recombinant protein expression in IPTG-induced bacterial host cells using the pET system.
