Peptide Coating Kit
Peptide Coating Kit
Brand: Takara Bio.
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Peptide Coating Kit
The free carboxyl groups of peptides used for plate coating can be covalently linked via reaction with the water-soluble peptide coupling reagent l-ethyl-3-(3-(dimethylamino)-propy1)carbodiimide (EDC). These peptides can subsequently be linked to amino groups that are attached to the surface of microtiter plate wells. The end result is a population of peptides directly coating the surface of the microtiter plate via peptide bonds.
The Peptide Coating Kit allows you to coat plates efficiently with low molecular weight proteins or synthetic peptides that are otherwise difficult to adsorb onto plates.
Applications
- Detection of antigen via ELISA
- Detection of antibody via competitive ELISA
Components
| Reaction plate | 96-well (8 well × 12 strips) × 5 plates |
|---|---|
| Coupling reagent | 1 vial (for 5 ml × 1) |
| Reaction buffer | 1 vial (50 ml × 1) |
| Blocking solution | 2 vials (50 ml × 2) |
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