pE2-Crimson is a prokaryotic expression vector which encodes E2-Crimson, our brightest and fastest-maturing far-red Living Colors fluorescent protein. E2-Crimson was derived from DsRed-Express2, and retains its fast maturation, high photostability, increased solubility, and low cytotoxicity. pE2-Crimson is primarily intended to serve as a source of E2-Crimson cDNA. The flanking MCS regions make it possible to excise the E2-Crimson coding sequence and insert it into other expression vectors of choice. The vector can also be used in bacteria to produce E2-Crimson protein.
Overview
Far-red fluorescent protein
E2-Crimson fluorescent protein excitation and emission maxima: 611 and 646 nm, respectively
Basic fluorescent protein vector map. Use this bacterial expression vector as a source of the fluorescent protein gene. Note: There is a stop codon at the 5' end of the 3' MCS. The 3' MCS should not be used for cloning.
E2-Crimson is useful for confocal and STED (stimulated emission depletion) microscopy
E2-Crimson is useful for confocal and STED (stimulated emission depletion) microscopy. The mammalian ER was imaged by conventional confocal microscopy (left) or by STED microscopy (right) with 635 nm excitation and a STED wavelength of 760 nm. The scale bar is 1 μm.
pE2-Crimson is a prokaryotic expression vector which encodes E2-Crimson, our brightest and fastest-maturing far-red Living Colors fluorescent protein. E2-Crimson was derived from DsRed-Express2, and retains its fast maturation, high photostability, increased solubility, and low cytotoxicity. pE2-Crimson is primarily intended to serve as a source of E2-Crimson cDNA. The flanking MCS regions make it possible to excise the E2-Crimson coding sequence and insert it into other expression vectors of choice. The vector can also be used in bacteria to produce E2-Crimson protein.
Overview
Far-red fluorescent protein
E2-Crimson fluorescent protein excitation and emission maxima: 611 and 646 nm, respectively
Basic fluorescent protein vector map. Use this bacterial expression vector as a source of the fluorescent protein gene. Note: There is a stop codon at the 5' end of the 3' MCS. The 3' MCS should not be used for cloning.
E2-Crimson is useful for confocal and STED (stimulated emission depletion) microscopy
E2-Crimson is useful for confocal and STED (stimulated emission depletion) microscopy. The mammalian ER was imaged by conventional confocal microscopy (left) or by STED microscopy (right) with 635 nm excitation and a STED wavelength of 760 nm. The scale bar is 1 μm.