pE2-Crimson-C1 Vector
pE2-Crimson-C1 Vector
Brand: Takara Bio.
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pE2-Crimson-C1 Vector
pE2-Crimson-C1 is a mammalian expression vector designed to express a protein of interest fused to the C-terminus of E2-Crimson, our brightest and fastest-maturing far-red Living Colors fluorescent protein. E2-Crimson was derived from DsRed-Express2, and retains its fast maturation, high photostability, increased solubility, and low cytotoxicity. The unmodified vector can be used to express E2-Crimson in mammalian cells. If required, stable transfectants can be selected using G418.
Overview
- Far-red fluorescent protein
- E2-Crimson fluorescent protein excitation and emission maxima: 611 and 646 nm, respectively
- Several of our red fluorescent protein antibodies strongly detect E2-Crimson
Applications
- Applications involving sensitive cells, such as primary cells and stem cells
- Fusions
- Protein localization studies
- General reporter for mammalian cells
- Monitoring transfection efficiencies
- In vivo imaging
Schematic for fluorescent fusion protein vectors
Schematic for fluorescent fusion protein vectors. Panel A. N-terminal fluorescent protein (FP) vector. Panel B. C-terminal fluorescent protein vector.
E2-Crimson is useful for confocal and STED (stimulated emission depletion) microscopy
E2-Crimson is useful for confocal and STED (stimulated emission depletion) microscopy. The mammalian ER was imaged by conventional confocal microscopy (left) or by STED microscopy (right) with 635 nm excitation and a STED wavelength of 760 nm. The scale bar is 1 μm.
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Schematic for fluorescent fusion protein vectors
Schematic for fluorescent fusion protein vectors. Panel A. N-terminal fluorescent protein (FP) vector. Panel B. C-terminal fluorescent protein vector.
E2-Crimson is useful for confocal and STED (stimulated emission depletion) microscopy
E2-Crimson is useful for confocal and STED (stimulated emission depletion) microscopy. The mammalian ER was imaged by conventional confocal microscopy (left) or by STED microscopy (right) with 635 nm excitation and a STED wavelength of 760 nm. The scale bar is 1 μm.
