pDsRed2 encodes DsRed2, a variant of the Discosoma sp. red fluorescent protein (DsRed). DsRed2 contains six amino acid substitutions that accelerate chromophore maturation and decrease protein aggregation. In addition, the coding sequence for DsRed2 contains a series of silent base-pair changes for higher expression in mammalian cells. In pDsRed2, the DsRed2 coding sequence is flanked by two separate MCS regions at the 5' and 3' ends, making it possible to excise and shuttle the DsRed2 coding region into other expression vectors. pDsRed2 is intended to serve as a source of the DsRed2 cDNA; It can also be used in bacteria to produce DsRed2 protein.
Basic fluorescent protein vector map. Use this bacterial expression vector as a source of the fluorescent protein gene. Note: There is a stop codon at the 5' end of the 3' MCS. The 3' MCS should not be used for cloning.
Excitation and emission spectra of reef coral fluorescent proteins AmCyan1, ZsGreen1, ZsYellow1, DsRed2, AsRed2, and HcRed1
Excitation and emission spectra of reef coral fluorescent proteins AmCyan1, ZsGreen1, ZsYellow1, DsRed2, AsRed2, and HcRed1.
pDsRed2 encodes DsRed2, a variant of the Discosoma sp. red fluorescent protein (DsRed). DsRed2 contains six amino acid substitutions that accelerate chromophore maturation and decrease protein aggregation. In addition, the coding sequence for DsRed2 contains a series of silent base-pair changes for higher expression in mammalian cells. In pDsRed2, the DsRed2 coding sequence is flanked by two separate MCS regions at the 5' and 3' ends, making it possible to excise and shuttle the DsRed2 coding region into other expression vectors. pDsRed2 is intended to serve as a source of the DsRed2 cDNA; It can also be used in bacteria to produce DsRed2 protein.
Basic fluorescent protein vector map. Use this bacterial expression vector as a source of the fluorescent protein gene. Note: There is a stop codon at the 5' end of the 3' MCS. The 3' MCS should not be used for cloning.
Excitation and emission spectra of reef coral fluorescent proteins AmCyan1, ZsGreen1, ZsYellow1, DsRed2, AsRed2, and HcRed1
Excitation and emission spectra of reef coral fluorescent proteins AmCyan1, ZsGreen1, ZsYellow1, DsRed2, AsRed2, and HcRed1.