pDsRed2-N1 encodes DsRed2, a variant of Discosoma sp. red fluorescent protein, (DsRed). DsRed2 contains six amino acid substitutions that accelerate chromophore maturation and decrease protein aggregation. In addition, the coding sequence for DsRed2 is human codon-optimized for higher expression in mammalian cells. pDsRed2-N1 allows genes to be cloned into the multiple cloning site (MCS) upstream of the DsRed2 coding sequence to be expressed as fusions to the N-terminus of DsRed2. The unmodified vector will express DsRed2 in mammalian cells.
Schematic for fluorescent fusion protein vectors.Panel A. N-terminal fluorescent protein (FP) vector. Panel B. C-terminal fluorescent protein vector.
Hygromycin DsRed-Monomer and AcGFP1 expression vectors
Hygromycin DsRed-Monomer and AcGFP1 expression vectors. Vector maps of our DsRed-Monomer and pAcGFP1 Hyg-N1 (Panel A) and Hyg-C1 (Panel B) mammalian expression vectors for fusion applications. Each vector contains a hygromycin selection cassette. These vectors can be used together with our standard N1/C1 vectors containing the neomycin selection cassette to establish double stable cell lines expressing two different Living Colors proteins.
Excitation and emission spectra of reef coral fluorescent proteins AmCyan1, ZsGreen1, ZsYellow1, DsRed2, AsRed2, and HcRed1
Excitation and emission spectra of reef coral fluorescent proteins AmCyan1, ZsGreen1, ZsYellow1, DsRed2, AsRed2, and HcRed1.
pDsRed2-N1 encodes DsRed2, a variant of Discosoma sp. red fluorescent protein, (DsRed). DsRed2 contains six amino acid substitutions that accelerate chromophore maturation and decrease protein aggregation. In addition, the coding sequence for DsRed2 is human codon-optimized for higher expression in mammalian cells. pDsRed2-N1 allows genes to be cloned into the multiple cloning site (MCS) upstream of the DsRed2 coding sequence to be expressed as fusions to the N-terminus of DsRed2. The unmodified vector will express DsRed2 in mammalian cells.
Schematic for fluorescent fusion protein vectors.Panel A. N-terminal fluorescent protein (FP) vector. Panel B. C-terminal fluorescent protein vector.
Hygromycin DsRed-Monomer and AcGFP1 expression vectors
Hygromycin DsRed-Monomer and AcGFP1 expression vectors. Vector maps of our DsRed-Monomer and pAcGFP1 Hyg-N1 (Panel A) and Hyg-C1 (Panel B) mammalian expression vectors for fusion applications. Each vector contains a hygromycin selection cassette. These vectors can be used together with our standard N1/C1 vectors containing the neomycin selection cassette to establish double stable cell lines expressing two different Living Colors proteins.
Excitation and emission spectra of reef coral fluorescent proteins AmCyan1, ZsGreen1, ZsYellow1, DsRed2, AsRed2, and HcRed1
Excitation and emission spectra of reef coral fluorescent proteins AmCyan1, ZsGreen1, ZsYellow1, DsRed2, AsRed2, and HcRed1.