pDsRed2-C1 encodes DsRed2, a variant of the Discosoma sp. red fluorescent protein (DsRed) that has been engineered for faster maturation and lower, non-specific aggregation. DsRed2 contains six amino acid substitutions that accelerate chromophore formation and decrease protein aggregation. In addition, the coding sequence for DsRed2 is human codon-optimized for high expression in mammalian cells. pDsRed2-C1 allows genes cloned into the multiple cloning site (MCS) downstream of the DsRed2 coding sequence to be expressed as fusions to the C-terminus of DsRed2 in mammalian cells.
Schematic for fluorescent fusion protein vectors.Panel A. N-terminal fluorescent protein (FP) vector. Panel B. C-terminal fluorescent protein vector.
Hygromycin DsRed-Monomer and AcGFP1 expression vectors
Hygromycin DsRed-Monomer and AcGFP1 expression vectors. Vector maps of our DsRed-Monomer and pAcGFP1 Hyg-N1 (Panel A) and Hyg-C1 (Panel B) mammalian expression vectors for fusion applications. Each vector contains a hygromycin selection cassette. These vectors can be used together with our standard N1/C1 vectors containing the neomycin selection cassette to establish double stable cell lines expressing two different Living Colors proteins.
pDsRed2-C1 encodes DsRed2, a variant of the Discosoma sp. red fluorescent protein (DsRed) that has been engineered for faster maturation and lower, non-specific aggregation. DsRed2 contains six amino acid substitutions that accelerate chromophore formation and decrease protein aggregation. In addition, the coding sequence for DsRed2 is human codon-optimized for high expression in mammalian cells. pDsRed2-C1 allows genes cloned into the multiple cloning site (MCS) downstream of the DsRed2 coding sequence to be expressed as fusions to the C-terminus of DsRed2 in mammalian cells.
Schematic for fluorescent fusion protein vectors.Panel A. N-terminal fluorescent protein (FP) vector. Panel B. C-terminal fluorescent protein vector.
Hygromycin DsRed-Monomer and AcGFP1 expression vectors
Hygromycin DsRed-Monomer and AcGFP1 expression vectors. Vector maps of our DsRed-Monomer and pAcGFP1 Hyg-N1 (Panel A) and Hyg-C1 (Panel B) mammalian expression vectors for fusion applications. Each vector contains a hygromycin selection cassette. These vectors can be used together with our standard N1/C1 vectors containing the neomycin selection cassette to establish double stable cell lines expressing two different Living Colors proteins.