pDsRed-Monomer-Golgi Vector
pDsRed-Monomer-Golgi Vector
Brand: Takara Bio.
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pDsRed-Monomer-Golgi Vector
pDsRed-Monomer-Golgi encodes DsRed-Monomer, a monomeric mutant of the Discosoma sp. red fluorescent protein DsRed. In this vector, the DsRed-Monomer coding sequence is fused at its N-terminus to the N-terminal 81 amino acids of human beta 1,4-galactosyltransferase (GT). The pDsRed-Monomer-Golgi fusion protein can be used for specific labeling of the trans-medial region of the Golgi apparatus in mammalian cells. A membrane anchoring signal peptide within the 81 amino acid sequence targets the fusion protein.
DsRed-Monomer is soluble when expressed in mammalian cells
DsRed-Monomer is soluble when expressed in mammalian cells. HeLa cells were transfected with pDsRed-Monomer-N1 and fixed in 4% paraformaldehyde 24 hr post-transfection. DsRed-Monomer fluorescent protein displays an even, consistent, and homogeneous distribution.
Organelles targeted by the subcellular localization vectors
Organelles targeted by the subcellular localization vectors.
Subcellular localization vectors encode fusions of fluorescent proteins with localization signals or subcellular structural proteins, which target the fluorescent protein to a specific organelle or subcellular structure
Subcellular localization vectors encode fusions of fluorescent proteins with localization signals or subcellular structural proteins, which target the fluorescent protein to a specific organelle or subcellular structure. The vectors are available in a variety of organelle- and cytoskeleton-targeted color variants.
DsRed-Monomer is a monomeric protein
DsRed-Monomer is a monomeric protein. Panel A. Recombinant DsRed-Express and DsRed-Monomer fluorescent proteins (100 μg) were analyzed by FPLC gel filtration chromatography. Overall absorbance (A280) and chromophore excitation (A557) of the eluted material were monitored simultaneously. DsRed-Monomer elutes from the column at a retention time (39 min) corresponding to a molecular weight of 28 kDa. The calculated molecular weight of DsRed-Monomer is 26.8 kDa. DsRed-Express is a tetrameric protein that elutes at an earlier retention time (33 min) corresponding to a molecular weight of 89 kDa. Panel B. Pseudonative gel analysis of proteins. The oligomeric structure of proteins is preserved during SDS PAGE analysis if samples are kept at 4°C and not boiled prior to loading on a gel. Boiled and unboiled recombinant proteins (7.5 μg) were separated by SDS PAGE electrophoresis (12% acrylamide). In both the boiled (denatured) and unboiled (nondenatured) samples, DsRed-Monomer fluorescent protein runs as a uniform band of ~30 kDa due to its monomeric structure. The unboiled (nondenatured) DsRed-Express runs at a much higher molecular weight than its boiled (denatured) counterpart due to its tetrameric structure.
Fluorescence excitation and emission spectra of DsRed-Monomer and AcGFP1
Fluorescence excitation and emission spectra of DsRed-Monomer and AcGFP1.
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DsRed-Monomer is soluble when expressed in mammalian cells
DsRed-Monomer is soluble when expressed in mammalian cells. HeLa cells were transfected with pDsRed-Monomer-N1 and fixed in 4% paraformaldehyde 24 hr post-transfection. DsRed-Monomer fluorescent protein displays an even, consistent, and homogeneous distribution.
Organelles targeted by the subcellular localization vectors
Organelles targeted by the subcellular localization vectors.
Subcellular localization vectors encode fusions of fluorescent proteins with localization signals or subcellular structural proteins, which target the fluorescent protein to a specific organelle or subcellular structure
Subcellular localization vectors encode fusions of fluorescent proteins with localization signals or subcellular structural proteins, which target the fluorescent protein to a specific organelle or subcellular structure. The vectors are available in a variety of organelle- and cytoskeleton-targeted color variants.
DsRed-Monomer is a monomeric protein
DsRed-Monomer is a monomeric protein. Panel A. Recombinant DsRed-Express and DsRed-Monomer fluorescent proteins (100 μg) were analyzed by FPLC gel filtration chromatography. Overall absorbance (A280) and chromophore excitation (A557) of the eluted material were monitored simultaneously. DsRed-Monomer elutes from the column at a retention time (39 min) corresponding to a molecular weight of 28 kDa. The calculated molecular weight of DsRed-Monomer is 26.8 kDa. DsRed-Express is a tetrameric protein that elutes at an earlier retention time (33 min) corresponding to a molecular weight of 89 kDa. Panel B. Pseudonative gel analysis of proteins. The oligomeric structure of proteins is preserved during SDS PAGE analysis if samples are kept at 4°C and not boiled prior to loading on a gel. Boiled and unboiled recombinant proteins (7.5 μg) were separated by SDS PAGE electrophoresis (12% acrylamide). In both the boiled (denatured) and unboiled (nondenatured) samples, DsRed-Monomer fluorescent protein runs as a uniform band of ~30 kDa due to its monomeric structure. The unboiled (nondenatured) DsRed-Express runs at a much higher molecular weight than its boiled (denatured) counterpart due to its tetrameric structure.
Fluorescence excitation and emission spectra of DsRed-Monomer and AcGFP1
Fluorescence excitation and emission spectra of DsRed-Monomer and AcGFP1.
