pDsRed-Express2-N1 Vector
pDsRed-Express2-N1 Vector
Brand: Takara Bio.
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pDsRed-Express2-N1 Vector
pDsRed-Express2-N1 is a mammalian expression vector that encodes DsRed-Express2, a variant of Discosoma sp. red fluorescent protein (DsRed). pDsRed-Express2-N1 can be used to construct fusions to the N-terminus of DsRed-Express2. The fusion construct retains the fluorescent properties of the native DsRed-Express2 protein, and its expression can be monitored by flow cytometry and its cellular localization can be determined by fluorescence microscopy. pDsRed-Express2-N1 can also be used as a cotransfection marker; the unmodified vector will express DsRed-Express2 in mammalian cells.
Robust expression of DsRed-Express2 in mouse bone marrow hematopoietic stem and progenitor cells
Robust expression of DsRed-Express and DsRed-Express2 in mouse bone marrow hematopoietic stem and progenitor cells. Mononuclear bone marrow cells were transduced with retroviral vectors encoding DsRed-Express (Panel A), DsRed-Express2 (Panel B), or EGFP (Panel C), and fluorescent cells were sorted 87 hr later. Red and green fluorescence signals were detected using the PE and FITC filter sets, respectively. The lines represent gates defined by analyzing untransduced cells.
Schematic for fluorescent fusion protein vectors
Schematic for fluorescent fusion protein vectors. Panel A. N-terminal fluorescent protein (FP) vector. Panel B. C-terminal fluorescent protein vector.
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Robust expression of DsRed-Express2 in mouse bone marrow hematopoietic stem and progenitor cells
Robust expression of DsRed-Express and DsRed-Express2 in mouse bone marrow hematopoietic stem and progenitor cells. Mononuclear bone marrow cells were transduced with retroviral vectors encoding DsRed-Express (Panel A), DsRed-Express2 (Panel B), or EGFP (Panel C), and fluorescent cells were sorted 87 hr later. Red and green fluorescence signals were detected using the PE and FITC filter sets, respectively. The lines represent gates defined by analyzing untransduced cells.
Schematic for fluorescent fusion protein vectors
Schematic for fluorescent fusion protein vectors. Panel A. N-terminal fluorescent protein (FP) vector. Panel B. C-terminal fluorescent protein vector.
