pDsRed-Express-N1 encodes DsRed-Express, a variant of Discosoma sp. red fluorescent protein (DsRed). DsRed-Express contains nine amino acid substitutions (as compared to DsRed1), which improve the solubility, increase the maturation rate, and decrease the level of residual green emission. pDsRed-Express allows genes cloned into the multiple cloning site (MCS) upstream of the DsRed-Express coding sequence to be expressed as fusions to the N-terminus of DsRed-Express. The unmodified vector will express DsRed-Express in mammalian cells.
Schematic for fluorescent fusion protein vectors.Panel A. N-terminal fluorescent protein (FP) vector. Panel B. C-terminal fluorescent protein vector.
Hygromycin DsRed-Monomer and AcGFP1 expression vectors
Hygromycin DsRed-Monomer and AcGFP1 expression vectors. Vector maps of our DsRed-Monomer and pAcGFP1 Hyg-N1 (Panel A) and Hyg-C1 (Panel B) mammalian expression vectors for fusion applications. Each vector contains a hygromycin selection cassette. These vectors can be used together with our standard N1/C1 vectors containing the neomycin selection cassette to establish double stable cell lines expressing two different Living Colors proteins.
pDsRed-Express-N1 encodes DsRed-Express, a variant of Discosoma sp. red fluorescent protein (DsRed). DsRed-Express contains nine amino acid substitutions (as compared to DsRed1), which improve the solubility, increase the maturation rate, and decrease the level of residual green emission. pDsRed-Express allows genes cloned into the multiple cloning site (MCS) upstream of the DsRed-Express coding sequence to be expressed as fusions to the N-terminus of DsRed-Express. The unmodified vector will express DsRed-Express in mammalian cells.
Schematic for fluorescent fusion protein vectors.Panel A. N-terminal fluorescent protein (FP) vector. Panel B. C-terminal fluorescent protein vector.
Hygromycin DsRed-Monomer and AcGFP1 expression vectors
Hygromycin DsRed-Monomer and AcGFP1 expression vectors. Vector maps of our DsRed-Monomer and pAcGFP1 Hyg-N1 (Panel A) and Hyg-C1 (Panel B) mammalian expression vectors for fusion applications. Each vector contains a hygromycin selection cassette. These vectors can be used together with our standard N1/C1 vectors containing the neomycin selection cassette to establish double stable cell lines expressing two different Living Colors proteins.