pDendra2 Vector
pDendra2 encodes Dendra2, a green-to-red photoswitchable fluorescent protein derived from the octocoral Dendronephthya sp. In this vector, the Dendra2 coding sequence can be excised using BamHI and HindIII, for transfer into another vector. The pDendra2 vector also contains a lac promoter sequence, and therefore can be used to express Dendra2 in E. coli. It is primarily intended to serve as a source of Dendra2 cDNA.
Overview
- Living Colors Dendra2 is a green-to-red, high-contrast photoswitchable fluorescent protein
- Irreversible switching from green to red is activated by UV or blue light
- Switched (red) form is highly photostable
- Matures at a wide range of temperatures
- Monomeric fluorescent protein; suitable for fusions
Applications
- Tracking cell, organelle, and protein movement
- Visualizing real-time protein dynamics (movement, degradation, etc.)
- Monitoring selective cell fate
- Determining protein half-life
Basic fluorescent protein vector map
Basic fluorescent protein vector map. Use this bacterial expression vector as a source of the fluorescent protein gene. Note: There is a stop codon at the 5' end of the 3' MCS. The 3' MCS should not be used for cloning.
Green-to-red photoconversion of Dendra2-tagged proteins
Green-to-red photoconversion of Dendra2-tagged proteins. HeLa cells were transiently transfected with vectors encoding Dendra2-tagged fusion proteins. Dendra2 was converted to the red state in selected cells by brief illumination with a 488 nm laser. Confocal images were taken in the green and red channels after photoconversion.
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Basic fluorescent protein vector map
Basic fluorescent protein vector map. Use this bacterial expression vector as a source of the fluorescent protein gene. Note: There is a stop codon at the 5' end of the 3' MCS. The 3' MCS should not be used for cloning.
Green-to-red photoconversion of Dendra2-tagged proteins
Green-to-red photoconversion of Dendra2-tagged proteins. HeLa cells were transiently transfected with vectors encoding Dendra2-tagged fusion proteins. Dendra2 was converted to the red state in selected cells by brief illumination with a 488 nm laser. Confocal images were taken in the green and red channels after photoconversion.
