pDendra2-C Vector
pDendra2-C Vector
Brand: Takara Bio.
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pDendra2-C Vector
pDendra2-C is a mammalian expression vector that encodes Dendra2, a green-to-red photoswitchable fluorescent protein derived from the octocoral Dendronephthya sp. An MCS at the 3' end of the coding sequence makes it possible to insert a gene of interest in order to express it as a C-terminal fusion with Dendra2. The unmodified vector can be used to express Dendra2 in mammalian cells.
Overview
- Living Colors Dendra2 is a green-to-red, high-contrast photoswitchable fluorescent protein
- Irreversible switching from green to red is activated by UV or blue light
- Switched (red) form is highly photostable
- Matures at a wide range of temperatures
- Monomeric fluorescent protein; suitable for fusions
Applications
- Tracking cell, organelle, and protein movement
- Visualizing real-time protein dynamics (movement, degradation, etc.)
- Monitoring selective cell fate
- Determining protein half-life
Green-to-red photoconversion of Dendra2-tagged proteins
Green-to-red photoconversion of Dendra2-tagged proteins. HeLa cells were transiently transfected with vectors encoding Dendra2-tagged fusion proteins. Dendra2 was converted to the red state in selected cells by brief illumination with a 488 nm laser. Confocal images were taken in the green and red channels after photoconversion.
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Green-to-red photoconversion of Dendra2-tagged proteins
Green-to-red photoconversion of Dendra2-tagged proteins. HeLa cells were transiently transfected with vectors encoding Dendra2-tagged fusion proteins. Dendra2 was converted to the red state in selected cells by brief illumination with a 488 nm laser. Confocal images were taken in the green and red channels after photoconversion.
