p53 Dominant-Negative Vector Set
p53 Dominant-Negative Vector Set
Brand: Takara Bio.
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p53 Dominant-Negative Vector Set
Set of two vectors for studying p53 and related signaling pathways. pCMV-p53 expresses the wild-type p53 tumor suppressor protein. pCMV-p53mt135 contains the gene encoding a dominant-negative mutation of p53 that blocks normal p53 activity. Both genes are expressed from the constitutive CMV promoter. Use these vectors in mammalian systems for studying p53 function in apoptosis, cell growth, and DNA damage repair.
Overview
- Simplify the complexities of signal transduction in vivo
- Dissect the network of signal transduction pathways
- Screen for compounds that activate key pathways
- Combine with cis reporter vectors to create a complete assay system
Signal transduction kits are available for in vivo studies of signal transduction—from signaling cascades to gene transcription
Signal transduction kits are available for in vivo studies of signal transduction—from signaling cascades to gene transcription.
Inhibition of NFκB activity using pCMV-IκBα and pCMVκIαBM from the IκB Dominant-Negative Vector Set
Inhibition of NFκB activity using pCMV-IκBα and pCMVκIαBM from the IκB Dominant-Negative Vector Set (Cat. # 631923). HEK 293 cells were transiently transfected with the indicated plasmids. After 16 hr, fresh medium was added to the cells along with TNF to induce the NFκB pathway. SEAP activity was assayed 6 hr later to determine NFκB activity.
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Signal transduction kits are available for in vivo studies of signal transduction—from signaling cascades to gene transcription
Signal transduction kits are available for in vivo studies of signal transduction—from signaling cascades to gene transcription.
Inhibition of NFκB activity using pCMV-IκBα and pCMVκIαBM from the IκB Dominant-Negative Vector Set
Inhibition of NFκB activity using pCMV-IκBα and pCMVκIαBM from the IκB Dominant-Negative Vector Set (Cat. # 631923). HEK 293 cells were transiently transfected with the indicated plasmids. After 16 hr, fresh medium was added to the cells along with TNF to induce the NFκB pathway. SEAP activity was assayed 6 hr later to determine NFκB activity.
