One-Step RNA PCR Kit
One-Step RNA PCR Kit
Brand: Takara Bio.
In stock
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One-Step RNA PCR Kit
Takara Bio's One Step RNA PCR Kit offers the convenience and time savings of performing reverse transcription and PCR in a single tube, without the need for changing buffers or adding reagents, minimizing the risk of contamination. AMV Reverse Transcriptase XL provides greater thermostability than other reverse transcriptases, and retains its activity over a wider range of reaction temperatures. The kit also contains AMV-Optimized Taq DNA Polymerase for efficient amplification of the cDNA synthesized by AMV Reverse Transcriptase XL.
Applications
- One-step RT-PCR
- cDNA synthesis
RT-PCR Amplification from Total RNA using the One Step RNA PCR Kit
RT-PCR Amplification from Total RNA using the One Step RNA PCR Kit. RNA was isolated from HL60 cells and used as a template for RT-PCR with the One-Step RNA PCR Kit. The primers chosen amplified products of 1 kb (lane 1), 2 kb (lane 2), and 4.4 kb (lane 3). Lane M contains lambda-Hind III DNA Markers.
Components
| RR024A | |
| AMV Reverse Transcriptase XL* (5 U/µl) | 50 µl |
| AMV-Optimized Taq DNA Polymerase (5 U/µl) | 50 µl |
| RNase Inhibitor (40 U/µl) | 60 µl |
| RNase-Free dH2O (DEPC-treated) | 2 x 1 ml |
| 10X One-Step RNA PCR Buffer | 250 µl |
| dNTP Mixture (10 mM each) | 250 µl |
| MgCl2 (25 mM) | 500 µl |
| Control F-1 Primer (20 pmol/µl) | 25 µl |
| Control R-1 Primer (20 pmol/µl) | 25 µl |
| Positive control RNA (2 x 105 copies/µl) | 25 µl |
*Manufactured by Life Science Co.
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RT-PCR Amplification from Total RNA using the One Step RNA PCR Kit
RT-PCR Amplification from Total RNA using the One Step RNA PCR Kit. RNA was isolated from HL60 cells and used as a template for RT-PCR with the One-Step RNA PCR Kit. The primers chosen amplified products of 1 kb (lane 1), 2 kb (lane 2), and 4.4 kb (lane 3). Lane M contains lambda-Hind III DNA Markers.
Components
| RR024A | |
| AMV Reverse Transcriptase XL* (5 U/µl) | 50 µl |
| AMV-Optimized Taq DNA Polymerase (5 U/µl) | 50 µl |
| RNase Inhibitor (40 U/µl) | 60 µl |
| RNase-Free dH2O (DEPC-treated) | 2 x 1 ml |
| 10X One-Step RNA PCR Buffer | 250 µl |
| dNTP Mixture (10 mM each) | 250 µl |
| MgCl2 (25 mM) | 500 µl |
| Control F-1 Primer (20 pmol/µl) | 25 µl |
| Control R-1 Primer (20 pmol/µl) | 25 µl |
| Positive control RNA (2 x 105 copies/µl) | 25 µl |
*Manufactured by Life Science Co.
