One-step PrimeScript RT-PCR kit for real-time RT-PCR

Real-time RT-PCR is made easy with the One Step PrimeScript RT-PCR Kit (Perfect Real Time), a one-step real-time RT-PCR kit designed for real-time RT-PCR (qPCR) via probe-based qPCR assays such as 5' nuclease assays1. In this 5' nuclease assay real-time PCR (qPCR) kit, one-step real-time RT-PCR can be performed in a single tube, simplifying amplification and minimizing the risk of contamination. Amplified products are monitored in real time, so there is no need to verify them through electrophoresis following PCR. The sensitivity provided by the enzymes in this kit enables detection of very small amounts of RNA, including RNA derived from viral, rare, or precious samples.

This kit uses PrimeScript RTase, a modified, recombinant MMLV (Moloney Murine Leukemia Virus) reverse transcriptase that is verified to have low levels of RNase H and which exhibits an excellent extension rate and quick, full-length cDNA synthesis, as well as Takara Ex Taq HS, a high efficiency hot start PCR enzyme optimized for this one-step real-time RT-PCR kit.

Overview

• Enables accurate and rapid analysis of viral RNA or RNA derived from rare or precious sources.
• Contains Takara Ex Taq HS, a high-efficiency, hot-start PCR enzyme, for PCR.
• Buffer system is real-time PCR optimized, increasing amplification efficiency and detection sensitivity.

Applications

• One-step real-time RT-PCR
• Probe-based qPCR assays such as 5' nuclease assays and real-time PCR (qPCR) assays