Applications

• Poly(A) mRNA isolation from total RNA
• Clean up of in-vitro transcripts
• Direct purification of poly(A) mRNA from cells

Features

• Convenient processing by microcentrifugation using NucleoTrap® Microfilters
• Direct mRNA isolation from cells
• High quality poly(A) mRNA without degradation and DNA contamination

Specifications

• Technology: Affinity chromatography
• Format: Oligo(dT) latex bead suspension
• Fragment size: 50 nt–20 knt
• A260/A280: 1.9–2.1
• Elution volume: 10–20 μL
• Binding capacity: 0.25 μg poly(A) mRNA/μL oligo(dT) latex bead suspension
• Processing time: 30 min/6 preps

NucleoTrap® mRNA Mini

• Sample material: < 250 μg total RNA
• Typical yield: 10 μg mRNA

NucleoTrap® mRNA Midi

• Sample material: < 1000 μg total RNA
• Typical yield: 40 μg mRNA