Applications

• Rapid isolation of RNA from formalin-fixed, paraffin-embedded samples, e.g., colon carcinoma (unstained), colon carcinoma (hematoxylin stained), colon mucosa, adenocarcinoma of colon transversum, liver (rat/human), liver carcinoma (hematoxylin stained), lymph node, spleen (rat/human), pancreas (rat/human; diabetic/non-diabetic), placenta
• Isolation of RNA from fresh and archived FFPE samples
• Isolation of RNA from specimen on object slides (stained or unstained)
• Typical downstream application: RT-PCR

Features

• Blue colored Paraffin Dissolver (patented technology) included - very easy and convenient paraffin removal
• rDNase included – efficient on-column DNA removal
• High quality RNA for enhanced RT-PCR performance

Specifications

• Technology: Silica-membrane technology
• Format: Mini spin columns
• Sample material: 4–10 sections (10 μm, up to 6 cm2 tissue area), < 50 mg of tissue
• RNA yield: Depending on amount and quality of the sample
• Elution volume: 30–50 µL
• Preparation time: 70 min/6 preps (90 min including optional rDNase digest)
• Binding capacity: 200 µg