Applications

• RNA from plant cells and tissue
• RNA from filamentous fungi
• Typical downstream applications: real-time RT-PCR, gene expression profiling, Northern blotting, primer extension, array technology, RNase protection assays

Features

• Two alternative lysis buffers included – optimized lysis procedure
• rDNase included for on-column digestion – efficient removal of contaminating DNA
• NucleoSpin® Filters (shredders) included – efficient sample homogenization and reduction of viscosity
• Up to 70 μg ready-to-use RNA
• Parallel purification of genomic DNA possible by using the NucleoSpin® RNA/DNA Buffer Set

Specifications

• Technology: Silica-membrane technology
• Format: Mini spin columns
• Sample material: < 100 mg tissue
• Fragment size: > 200 b
• Typical yield: 3–70 µg
• A260/A280: 1.9–2.1
• Elution volume: 40–60 µL
• Preparation time: 30 min/6 preps
• Binding capacity: 200 µg