Applications

• RNA isolation from cultured cells, tissue (standard protocol)
• RNA from < 10¹⁰ bacterial cells (Gram-negative, Gram-positive) or < 3 x 10⁸ yeast cells
• RNA clean-up from reaction mixtures
• RNA from samples stored in RNAlater®
• Typical downstream applications: real-time RT-PCR, Northern blotting, primer extension, array technology, RNase protection assays

Features

• rDNase included for on-column digestion – easy and efficient removal of genomic DNA
• NucleoSpin® Filters Midi (shredders) – efficient sample homogenization and reduction of viscosity
• Up to 600 μg ready-to-use RNA

Specifications

• Technology: Silica membrane technology
• Fragment size: > 200 nt
• A260/A280: 1.9–2.1
• Processing: Centrifugation
• Sample material: Cultured cells (< 5 x 10⁷), bacterial cells (< 10¹⁰), yeast cells (< 3 x 10⁸), human / animal tissue (< 20 mg)
• Typical yield: 180 μg (107 HeLa cells), HeLa cells (4 x 10⁷): 620 μg
• Elution volume: 500–1000 μL
• Binding capacity: 700 μg
• Processing time: 80 min/4 preps