NucleoSpin RNA Blood Midi
NucleoSpin RNA Blood Midi
Brand: MACHEREY-NAGEL
In stock
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NucleoSpin RNA Blood Midi
Applications
- Isolation of RNA from fresh or frozen whole blood from common blood collection tubes (EDTA, citrate, heparin)
- Typical downstream applications: qRT-PCR, next generation sequencing, blotting, array technology
Features
- Direct total blood lysis – very simple and convenient procedure (patent pending)
- No selective erythrocyte lysis at 4 °C – complete processing at room temperature
- Superior RNA yield and quality from up to 1.3 mL whole blood
- Efficient on-column DNA removal for an increased sensitivity in downstream applications
- Compatible with common blood collection tubes and anticoagulants (e.g., EDTA, citrate, and heparin)
Specifications
- Technology: Silica membrane technology
- A260/A280: 1.9–2.1
- Fragment size: > 200 nt
- Processing: Centrifugation
- Sample material: 400–1300 μL blood
- Typical yield: Blood (1300 μL): 4–26 μg*
- Elution volume: 200–400 μL
- Binding capacity: 700 μg
- Processing time: 75 min/6 preps
The NucleoSpin RNA Blood procedure
The NucleoSpin RNA Blood procedure.
NucleoSpin RNA Blood and NucleoSpin RNA Blood Midi provide higher RNA yields from smaller whole blood samples than competitor kits
NucleoSpin RNA Blood and NucleoSpin RNA Blood Midi provide higher RNA yields from smaller whole blood samples than competitor kits. RNA was isolated from the indicated blood volumes following each manufacturer's protocol. The quality of isolated RNA is comparable. Average RIN numbers (data not shown): 7.5 (Q); 7.7 (NucleoSpin RNA Blood); 8.8 (NucleoSpin RNA Blood Midi); 8.9 (Q, PAXgene).
The NucleoSpin RNA Blood Midi procedure
The NucleoSpin RNA Blood Midi procedure.
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The NucleoSpin RNA Blood procedure
The NucleoSpin RNA Blood procedure.
NucleoSpin RNA Blood and NucleoSpin RNA Blood Midi provide higher RNA yields from smaller whole blood samples than competitor kits
NucleoSpin RNA Blood and NucleoSpin RNA Blood Midi provide higher RNA yields from smaller whole blood samples than competitor kits. RNA was isolated from the indicated blood volumes following each manufacturer's protocol. The quality of isolated RNA is comparable. Average RIN numbers (data not shown): 7.5 (Q); 7.7 (NucleoSpin RNA Blood); 8.8 (NucleoSpin RNA Blood Midi); 8.9 (Q, PAXgene).
The NucleoSpin RNA Blood Midi procedure
The NucleoSpin RNA Blood Midi procedure.
