NucleoSpin Plasmid Transfection-grade
NucleoSpin Plasmid Transfection-grade
Brand: MACHEREY-NAGEL
In stock
SKU
NucleoSpin Plasmid Transfection-grade
Applications
- High‑copy plasmid DNA purification from E. coli cultures
Features
- Novel technology to diminish endotoxin content (< 50 EU/μg DNA) for successful transfections (patent pending)
- Purification of transfection-grade DNA in mini-format simplifies your work-flow
- 96‑well version for high throughput applications available
Specifications
- Technology: Silica membrane technology and endotoxin reduction technology
- Endotoxin level: < 50 EU/μg DNA
- Sample material: < 5 mL
- Vector size: < 15 kbp
- Typical yield: 15–30 μg
- Elution volume: 30–50 μL
- Binding capacity: 35 μg
- Processing time: 14 min/6 preps
Cell compatibility of eluted DNA
A pCMV-GFP plasmid (kindly provided by PlasmidFactory GmbH & Co. KG, Bielefeld, Germany) was purified from E. coli using NucleoSpin® Plasmid Transfection-grade. Plasmids were transfected into HEK239 cells by lipofection (Lipofectamine 2000) or Nucleofection™ with > 90 % transfection efficiency in both cases. Cell survival was compared to controls without DNA addition. The results show that cell survival is not affected by DNA eluates purified with NucleoSpin® Plasmid Transfection-grade.
Endotoxin levels appropriate for individual applications
A quantitative chromogenic LAL-test was used to assess endotoxin content. As indicated, the content of endotoxin is strongly depended on the technology of plasmid purification. Low endotoxin levels were detected after purification with NucleoSpin® Plasmid Transfection-grade, resulting in a plasmid solution directly appropriate for transfection of common cells.
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Cell compatibility of eluted DNA
A pCMV-GFP plasmid (kindly provided by PlasmidFactory GmbH & Co. KG, Bielefeld, Germany) was purified from E. coli using NucleoSpin® Plasmid Transfection-grade. Plasmids were transfected into HEK239 cells by lipofection (Lipofectamine 2000) or Nucleofection™ with > 90 % transfection efficiency in both cases. Cell survival was compared to controls without DNA addition. The results show that cell survival is not affected by DNA eluates purified with NucleoSpin® Plasmid Transfection-grade.
Endotoxin levels appropriate for individual applications
A quantitative chromogenic LAL-test was used to assess endotoxin content. As indicated, the content of endotoxin is strongly depended on the technology of plasmid purification. Low endotoxin levels were detected after purification with NucleoSpin® Plasmid Transfection-grade, resulting in a plasmid solution directly appropriate for transfection of common cells.
