NucleoSpin Plasmid / Plasmid (No Lid)
NucleoSpin Plasmid / Plasmid (No Lid)
Brand: MACHEREY-NAGEL
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NucleoSpin Plasmid / Plasmid (No Lid)
Rapid purification of plasmid DNA using centrifugation or vacuum
Applications
- High- and low‑copy* plasmid DNA purification from E. coli cultures and Gram positive bacteria
- Plasmid DNA clean up from reaction mixtures
Features
- High capacity – up to 50 μg of plasmid DNA
- Flexible solutions for full automation available
- Optional washing step for highest plasmid DNA quality
Specifications
- Technology: Silica membrane technology
- Endotoxin level: >> 50 EU/μg DNA
- Processing: Manual (centrifugation or vacuum)
- Sample material: 2–5 mL (high‑copy plasmid), 5–10 mL (low‑copy plasmid)
- Vector size: < 25 kbp
- Typical yield: 25–45 μg
- Elution volume: 50 μL
- Binding capacity: 50 μg
- Processing time: 25 min/6 preps
High yield plasmid Mini prep
Plasmid DNA isolation (pUC18) from E. coli DH5α using NucleoSpin® Plasmid. For the isolation 2 mL (lane 1–2), 5 mL (lane 3–4) and 8 mL (lane 5–6) LB cultures were used and analyzed on an agarose gel (2 μL of each eluate). Lane M: marker.
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High yield plasmid Mini prep
Plasmid DNA isolation (pUC18) from E. coli DH5α using NucleoSpin® Plasmid. For the isolation 2 mL (lane 1–2), 5 mL (lane 3–4) and 8 mL (lane 5–6) LB cultures were used and analyzed on an agarose gel (2 μL of each eluate). Lane M: marker.
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