NucleoSpin Gel and PCR Clean-up
NucleoSpin Gel and PCR Clean-up
Brand: MACHEREY-NAGEL
In stock
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NucleoSpin Gel and PCR Clean-up
Applications
- Purification of PCR products
- Extraction of DNA / RNA from agarose and polyacrylamide gels
Features
- High recoveries for small fragments down to 50 bp
- Minimized elution volume of 15 μL – highly concentrated DNA
- Separate buffers for single stranded DNA / RNA or SDS containing samples available
Specifications
- Technology: Silica membrane technology
- Processing: Centrifugation or vacuum (elution in centrifuge)
- Sample material: PCR reaction mixture (< 400 μL), TAE / TBE agarose gel (< 400 mg)
- Fragment size: 50 bp–approx. 20 kbp
- Typical recovery: 70–95 %
- A260/A280: 1.8–1.9
- Elution volume: 15–30 μL
- Binding capacity: 25 μg
- Processing time: 10 min/6 preps
PCR clean up: High recovery down to 50 bp
High recovery rates were achieved for fragments down to 50 bp, demonstrating the ability to reliably purify even short fragments into as little as 15 µL of elution volume (15 µL elution volume in dark blue, 30 µL elution volume in blue, and 50 µL in light blue).
Gel extraction: High recovery for a wide range of fragment sizes
A high extraction yield was reliably reached across the fragment size span.
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PCR clean up: High recovery down to 50 bp
High recovery rates were achieved for fragments down to 50 bp, demonstrating the ability to reliably purify even short fragments into as little as 15 µL of elution volume (15 µL elution volume in dark blue, 30 µL elution volume in blue, and 50 µL in light blue).
Gel extraction: High recovery for a wide range of fragment sizes
A high extraction yield was reliably reached across the fragment size span.
