NucleoSpin Gel and PCR Clean-up Maxi
NucleoSpin Gel and PCR Clean-up Maxi
Brand: MACHEREY-NAGEL
In stock
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NucleoSpin Gel and PCR Clean-up Maxi
Applications
- Purification of PCR products
- Extraction of DNA / RNA from agarose and polyacrylamide gels
- Typical downstream applications: clining, sequencing, PCR, restriction analysis
- NucleoSpin gel and PCR Clean-up ensures complete removal of contaminations auch as:
- nucleoties, primers
- enzymes
- mineral oil
- PCR additives (e.g., saltes betaine, DMSO)
- detergents (e.g., Tween 20, Triton X-100)
- dyes (e.g., ethidiumbromide, crystal violet, Stain G, Midori Green, Roti® Safe GelStain, DNA SafeStain)
Features
- Two applications in one kit – one buffer with optimal performance for both applications
- High recoveries for fragments down to 50 bp
- Binding buffer with pH indicator
- Separate buffers for single stranded DNA or SDS containing samples available
- Suitable for all gel buffer systems (e.g., TAE, TBE)
Specifications
- Technology: Silica membrane technology
- Processing: Centrifugation
- Sample material: < 10 mL PCR reaction mixture, < 10 g TAE/TBE agarose gel
- Fragment size: 50 bp–approx. 20 kbp
- Typical recovery: 70–95 %
- A260/A280: 1.75–1.85
- Elution volume: 1000 µL
- Binding capacity: 250 µg
- Processing time: 30 min/6 preps
PCR clean up: High recovery down to 50 bp
High recovery rates were achieved for fragments down to 50 bp, demonstrating the ability to reliably purify even short fragments into as little as 15 µL of elution volume (15 µL elution volume in dark blue, 30 µL elution volume in blue, and 50 µL in light blue).
Gel extraction: High recovery for a wide range of fragment sizes
A high extraction yield was reliably reached across the fragment size span.
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PCR clean up: High recovery down to 50 bp
High recovery rates were achieved for fragments down to 50 bp, demonstrating the ability to reliably purify even short fragments into as little as 15 µL of elution volume (15 µL elution volume in dark blue, 30 µL elution volume in blue, and 50 µL in light blue).
Gel extraction: High recovery for a wide range of fragment sizes
A high extraction yield was reliably reached across the fragment size span.
