NucleoSpin 96 Plasmid Transfection-grade Kits / Core Kits
NucleoSpin 96 Plasmid Transfection-grade Kits / Core Kits
Brand: MACHEREY-NAGEL
In stock
SKU
NucleoSpin 96 Plasmid Transfection-grade Kits / Core Kits
Applications
- Manual and automated isolation of high-copy plasmids
- Typical downstream applications: transfection of common cell lines, cell-based assays, screenings
Features
- Novel technology to diminish endotoxin content (< 50 EU/µg DNA) for successful transfections
- Innovative MN Wash Plate minimizes risk of cross-contamination
- NucleoSpin® 96 Plasmid Transfection-grade Core Kits*: Kits with basic content focusing on automation platforms. Additional accessories can be combined as needed.
Specifications
- Technology: Silica-membrane and endotoxin-reduction technology
- Format: 96-well plate
- Processing: Manual or automated, vacuum or centrifugation
- Endotoxin content: < 50 EU/µg DNA (EU=Endotoxin Units)
- Sample material: Up to 5 mL bacterial culture (E. coli, high-copy plasmids)
- Vector size: < 15 kbp
- Typical yield: 5–20 µg
- A260/A280: 1.80–1.85
- Elution volume: 100–200 µL
- Preparation time: 45 min/plate
- Binding capacity: 20 µg
Reliable yields across purification platforms
NucleoSpin® 96 Plasmid Transfection-grade was used to isolate plasmid DNA from 1.5 mL of bacterial cultures (E. coli DH5α™, carrying a high copy plasmid pGEMR-T Easy, n=24, with a 982 bp insert) on a positive pressure module ([MPE]2) or a manual vacuum manifold (vacuum). Regardless of the technology applied, NucleoSpin® 96 Plasmid Transfection-grade kit delivered reliably high yields with low variation.
Reliable purity both with vacuum chamber and a positive pressure unit
NucleoSpin® 96 Plasmid Transfection-grade was used to isolate plasmid DNA from 1.5 mL of bacterial cultures (E. coli DH5α™, carrying a high copy plasmid pGEMR-T Easy, n=24, with a 982 bp insert) on a positive pressure module ([MPE]2) or a manual vacuum manifold (Vacuum). Very similar purity levels as indicated by the A280/A260 and A260/A260 optical measurements indicate the reliably high purity of plasmid preparations with the kit, even when combined with two different technologies.
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Reliable yields across purification platforms
NucleoSpin® 96 Plasmid Transfection-grade was used to isolate plasmid DNA from 1.5 mL of bacterial cultures (E. coli DH5α™, carrying a high copy plasmid pGEMR-T Easy, n=24, with a 982 bp insert) on a positive pressure module ([MPE]2) or a manual vacuum manifold (vacuum). Regardless of the technology applied, NucleoSpin® 96 Plasmid Transfection-grade kit delivered reliably high yields with low variation.
Reliable purity both with vacuum chamber and a positive pressure unit
NucleoSpin® 96 Plasmid Transfection-grade was used to isolate plasmid DNA from 1.5 mL of bacterial cultures (E. coli DH5α™, carrying a high copy plasmid pGEMR-T Easy, n=24, with a 982 bp insert) on a positive pressure module ([MPE]2) or a manual vacuum manifold (Vacuum). Very similar purity levels as indicated by the A280/A260 and A260/A260 optical measurements indicate the reliably high purity of plasmid preparations with the kit, even when combined with two different technologies.
