NucleoSpin 8 / 96 Plasmid Kits and Core Kits
NucleoSpin 8 / 96 Plasmid Kits and Core Kits
Brand: MACHEREY-NAGEL
In stock
SKU
NucleoSpin 8 / 96 Plasmid Kits and Core Kits
Applications
- Manual and automated isolation of high-copy plasmids
- Support protocol available for purification of cosmids
- Typical downstream applications: cloning, sequencing, PCR, transformation, restriction analysis
Features
- Time-saving parallel isolation of plasmid DNA
- Processing of up to 5 mL bacterial culture
- NucleoSpin® 8 Filter Strips/96 Filter Plate for convenient filtration of bacterial lysates
- Processing under vacuum or by centrifugation
- Suitable for manual or automated processing under vacuum
- Innovative MN Wash Plate minimizes risk of cross-contamination
- NucleoSpin® 8 / 96 Plasmid Core Kits: Kits with basic content focusing on automation platforms. Additional accessories can be combined as needed.
Specifications
- Technology: Silica membrane technology
- Endotoxin level: >> 50 EU/μg DNA
NucleoSpin® 8 Plasmid
- Processing: Manual or automated
- Sample material: 1–5 mL
- Vector size: < 15 kbp
- Typical yield: 5–20 μg
- Elution volume: 75–150 μL
- Binding capacity: 20 μg
- Processing time: 45 min/48 preps
NucleoSpin® 96 Plasmid
- Processing: Manual or automated
- Sample material: 1–5 mL
- Vector size: < 15 kbp
- Typical yield: 5–20 μg
- Elution volume: 75–150 μL
- Binding capacity: 20 μg
- Processing time: 45 min/96 preps
High yield plasmid Mini prep
Plasmid DNA isolation (pUC18) from E. coli DH5α using NucleoSpin® Plasmid. For the isolation 2 mL (lane 1–2), 5 mL (lane 3–4) and 8 mL (lane 5–6) LB cultures were used and analyzed on an agarose gel (2 μL of each eluate). Lane M: marker.
Isolation of plasmid DNA from bacterial cultures on Hamilton [MPE]2
Plasmid DNA of two different bacterial strains, transformed with plasmids containing either a 1482 bp or a 982 bp inserts, was isolated from 1.5 mL of bacterial cultures (E. coli DH 5α™, high-copy plasmid pGEM®-T Easy; n=24) using the NucleoSpin® 96 Plasmid kit on a [MPE]² positive pressure module (dark blue) or a manual vacuum manifold (light blue). Total yield was determined by UV spectrometry showing comparable yields between positive pressure or vacuum processed samples.
Purity of isolated plasmid DNA from bacterial cultures
Plasmid DNA of two different bacterial strains, transformed with plasmids containing either a 1482 bp or a 982 bp inserts, was isolated from 1.5 mL of bacterial cultures (E. coli DH 5α, high copy plasmid pGEM®-T Easy; n=24) using the NucleoSpin® 96 Plasmid kit on a [MPE]² positive pressure module (A260/A280: dark blue bars; A260/A230: orange squares) or a manual vacuum manifold (A260/A280: light blue bars; A260/A230: orange squares). Purity was determined by UV spectrometry revealing comparable quality of positive pres‑ sure or vacuum processed samples.
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High yield plasmid Mini prep
Plasmid DNA isolation (pUC18) from E. coli DH5α using NucleoSpin® Plasmid. For the isolation 2 mL (lane 1–2), 5 mL (lane 3–4) and 8 mL (lane 5–6) LB cultures were used and analyzed on an agarose gel (2 μL of each eluate). Lane M: marker.
Isolation of plasmid DNA from bacterial cultures on Hamilton [MPE]2
Plasmid DNA of two different bacterial strains, transformed with plasmids containing either a 1482 bp or a 982 bp inserts, was isolated from 1.5 mL of bacterial cultures (E. coli DH 5α™, high-copy plasmid pGEM®-T Easy; n=24) using the NucleoSpin® 96 Plasmid kit on a [MPE]² positive pressure module (dark blue) or a manual vacuum manifold (light blue). Total yield was determined by UV spectrometry showing comparable yields between positive pressure or vacuum processed samples.
Purity of isolated plasmid DNA from bacterial cultures
Plasmid DNA of two different bacterial strains, transformed with plasmids containing either a 1482 bp or a 982 bp inserts, was isolated from 1.5 mL of bacterial cultures (E. coli DH 5α, high copy plasmid pGEM®-T Easy; n=24) using the NucleoSpin® 96 Plasmid kit on a [MPE]² positive pressure module (A260/A280: dark blue bars; A260/A230: orange squares) or a manual vacuum manifold (A260/A280: light blue bars; A260/A230: orange squares). Purity was determined by UV spectrometry revealing comparable quality of positive pres‑ sure or vacuum processed samples.
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