DNA isolation from whole blood (fresh, frozen, or stabilized), serum, plasma, buffy coat, platelets, body fluids (e.g., amniotic fluid), cultured cells
Specifications
Technology: Silica membrane technology
NucleoSpin® 8 Blood
NucleoSpin® 96 Blood
Processing: Manual or automated
Sample material: Blood (< 200 μL), human / animal cells (2 x 10⁶)
Fragment size: 300 bp–approx. 50 kbp
Typical yield: 4–6 μg
Elution volume: 100 μL
Binding capacity: 20 μg
Processing time: 35 min/48 preps
Processing: Manual or automated
Sample material: Blood (< 200 μL), human / animal cells (2 x 10⁶)
Fragment size: 300 bp–approx. 50 kbp
Typical yield: 4–6 μg
Elution volume: 100 μL
Binding capacity: 20 μg
Processing time: 70 min/96 preps
Principle / Procedure
NucleoSpin® 8 / 96 Blood is designed for the parallel isolation of genomic DNA from whole blood. After lysis of blood cells genomic DNA is selectively bound to a silica membrane while contaminants are washed away effectively. Elution is possible even under vacuum without cross-contamination. Due to its plate design and the possibility to perform the complete procedure under vacuum NucleoSpin® 8 / 96 Blood can be easily adapted to common liquid handling instruments for fully automated processing without manual interactions.
Required hardware
Vacuum processing The NucleoSpin® 8 / 96 Blood kits can be used manually with the NucleoVac 96 vacuum manifold. For vacuum processing of the 8-well Strips the reusable Starter Set A, containing Column Holders A and NucleoSpin® Dummy Strips is required. For automation on laboratory platforms with standard 96-well plate vacuum chambers the use of the Starter Set A is also required.
Purification of genomic DNA from whole blood with NucleoSpin® 8 Blood Genomic DNA has been purified from human blood (200 μL each, frozen for six weeks at -80 °C) using NucleoSpin® 8 Blood manually under vacuum. 18 μL out of 200 μL eluate have been analyzed on a 0.7 % TAE agarose gel. M: λHindIII
Purification of genomic DNA from animal blood with NucleoSpin® 96 Blood Genomic DNA was extracted manually from pig blood (50 μL, stabilized either with EDTA or citrate) using NucleoSpin® 96 Blood with an average yield of about 1.60 μg. 20 μL of the eluate were analyzed on a 0.7 % TAE agarose gel. M: λHindIII
Please contact your local MN distributor for technical support regarding hardware, software, setup instructions as well as selection and evaluation of the protocol. Ready-to-run protocols can be customized on request according to your needs.
Complete automation NucleoSpin® 96 Blood can be automated on common laboratory workstations. 96 aliquots from the same blood sample have been purified fully automated on a Tecan Genesis™. The average yield is 6.76 ± 0.71 μg with a coefficient of variation (CV) of 10 %. The average purity is 1.97 ± 0.02 (CV: 1 %).
High and consistent yield of genomic DNA Genomic DNA was prepared from 200 μL whole blood using NucleoSpin® 96 Blood on the Tecan Genesis™ fully automated under vacuum. Average yield is 6.76 ± 0.71 μg.
No cross-contamination Due to optimized plate geometry and binding membrane complete processing under vacuum is possible without cross-contaminations. Genomic DNA (48 samples, loaded in a chess-board pattern) was purified from 200 μL whole blood fully automated on a Biomek® 2000. All 96 eluates were used for subsequent PCR (35 cycles). A PCR product is only visible in those wells that contained whole blood in the beginning of the procedure (+). In the other lanes only the primer can be seen.
Removal of PCR inhibitors Genomic DNA from 200 μL blood (96 samples) was isolated on a Tecan Genesis™ 150 and used in LightCycler™ analysis. All samples show a CT value of 23. No inhibition of PCR reactions is observed.
DNA isolation from whole blood (fresh, frozen, or stabilized), serum, plasma, buffy coat, platelets, body fluids (e.g., amniotic fluid), cultured cells
Specifications
Technology: Silica membrane technology
NucleoSpin® 8 Blood
NucleoSpin® 96 Blood
Processing: Manual or automated
Sample material: Blood (< 200 μL), human / animal cells (2 x 10⁶)
Fragment size: 300 bp–approx. 50 kbp
Typical yield: 4–6 μg
Elution volume: 100 μL
Binding capacity: 20 μg
Processing time: 35 min/48 preps
Processing: Manual or automated
Sample material: Blood (< 200 μL), human / animal cells (2 x 10⁶)
Fragment size: 300 bp–approx. 50 kbp
Typical yield: 4–6 μg
Elution volume: 100 μL
Binding capacity: 20 μg
Processing time: 70 min/96 preps
Principle / Procedure
NucleoSpin® 8 / 96 Blood is designed for the parallel isolation of genomic DNA from whole blood. After lysis of blood cells genomic DNA is selectively bound to a silica membrane while contaminants are washed away effectively. Elution is possible even under vacuum without cross-contamination. Due to its plate design and the possibility to perform the complete procedure under vacuum NucleoSpin® 8 / 96 Blood can be easily adapted to common liquid handling instruments for fully automated processing without manual interactions.
Required hardware
Vacuum processing The NucleoSpin® 8 / 96 Blood kits can be used manually with the NucleoVac 96 vacuum manifold. For vacuum processing of the 8-well Strips the reusable Starter Set A, containing Column Holders A and NucleoSpin® Dummy Strips is required. For automation on laboratory platforms with standard 96-well plate vacuum chambers the use of the Starter Set A is also required.
Purification of genomic DNA from whole blood with NucleoSpin® 8 Blood Genomic DNA has been purified from human blood (200 μL each, frozen for six weeks at -80 °C) using NucleoSpin® 8 Blood manually under vacuum. 18 μL out of 200 μL eluate have been analyzed on a 0.7 % TAE agarose gel. M: λHindIII
Purification of genomic DNA from animal blood with NucleoSpin® 96 Blood Genomic DNA was extracted manually from pig blood (50 μL, stabilized either with EDTA or citrate) using NucleoSpin® 96 Blood with an average yield of about 1.60 μg. 20 μL of the eluate were analyzed on a 0.7 % TAE agarose gel. M: λHindIII
Please contact your local MN distributor for technical support regarding hardware, software, setup instructions as well as selection and evaluation of the protocol. Ready-to-run protocols can be customized on request according to your needs.
Complete automation NucleoSpin® 96 Blood can be automated on common laboratory workstations. 96 aliquots from the same blood sample have been purified fully automated on a Tecan Genesis™. The average yield is 6.76 ± 0.71 μg with a coefficient of variation (CV) of 10 %. The average purity is 1.97 ± 0.02 (CV: 1 %).
High and consistent yield of genomic DNA Genomic DNA was prepared from 200 μL whole blood using NucleoSpin® 96 Blood on the Tecan Genesis™ fully automated under vacuum. Average yield is 6.76 ± 0.71 μg.
No cross-contamination Due to optimized plate geometry and binding membrane complete processing under vacuum is possible without cross-contaminations. Genomic DNA (48 samples, loaded in a chess-board pattern) was purified from 200 μL whole blood fully automated on a Biomek® 2000. All 96 eluates were used for subsequent PCR (35 cycles). A PCR product is only visible in those wells that contained whole blood in the beginning of the procedure (+). In the other lanes only the primer can be seen.
Removal of PCR inhibitors Genomic DNA from 200 μL blood (96 samples) was isolated on a Tecan Genesis™ 150 and used in LightCycler™ analysis. All samples show a CT value of 23. No inhibition of PCR reactions is observed.
