NucleoSnap Plasmid Midi
NucleoSnap Plasmid Midi
Brand: MACHEREY-NAGEL
In stock
SKU
NucleoSnap Plasmid Midi
Applications
- High- and low‑copy plasmid DNA purification from E. coli cultures
Features
- New column design (snap off column) for vacuum processing of large sample volumes
- Isolate up to 700 μg plasmid DNA in only 35 minutes
- No need for time consuming DNA precipitation
Specifications
- Technology: Precipitation and filtration
- Processing: Vacuum processing, centrifugation for elution
- Endotoxin level: < 50 EU/μg DNA
- Sample material: ≤ 50 mL
- Vector size: < 25 kbp
- Typical yield: 400–700 μg
- Elution volume: 200–500 μL
- Binding capacity: 1.5 mg
- Processing time: 35 min/6 preps
Superior performance in electroporation experiment
Eukaryotic cells were manipulated with the Nucleofector™ technology. The viability of cells treated with plasmid DNA isolated with the NucleoSnap® Plasmid Midi kit is comparable to the viability of cells in the mock control (grey bar), indicating that the plasmid DNA does not affect cell viability. In contrast, Nucleofection™ with plasmid DNA isolated with the molecular biology-grade NucleoSpin® Plasmid kit leads to a decrease in cell viability.
Procedure
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Superior performance in electroporation experiment
Eukaryotic cells were manipulated with the Nucleofector™ technology. The viability of cells treated with plasmid DNA isolated with the NucleoSnap® Plasmid Midi kit is comparable to the viability of cells in the mock control (grey bar), indicating that the plasmid DNA does not affect cell viability. In contrast, Nucleofection™ with plasmid DNA isolated with the molecular biology-grade NucleoSpin® Plasmid kit leads to a decrease in cell viability.
Procedure
