NucleoMag RNA
Applications
- RNA isolation from cultured / bacterial / yeast cells (< 2 x 10⁶), human / animal tissue (< 20 mg)
Features
- Recombinant DNase included
- Reducing agent TCEP included, no β-mercaptoethanol necessary
- Suitable for manual and automated processing
Specifications
- Technology: Magnetic bead technology
- Processing: Manual or automated
- Sample material: Cells (< 2 x 10⁶), tissue (< 20 mg)
- Fragment size: < 200 kbp
- Typical yield: < 30 μg
- Elution volume: 50–200 μL
- Binding capacity: 0.3 μg/μL beads
- Processing time: 60 min/96 preps
Robust yields and excellent performance in downstream applications
DNA was isolated from fresh 200 μL human blood samples (n=12) using the NucleoMag® Blood 200 μL kit on an epMotion® 5073m workstation. The DNA concentration of all 12 samples was determined by UV spectroscopy, dark blue bars). Performance in downstream applications was evaluated by con‑ ducting qPCR for a 250 bp sequence in the ß-actin gene. The target sequence was successfully amplified in all samples (orange squares = CT values).
Highly pure nucleic acids from human blood samples
DNA was isolated from fresh 200 μL human blood samples (n=12) using the NucleoMag Blood 200 μL kit on a epMotion® 5073m workstation. The purity was determined by UV spectroscopy. DNA quality analysis resulted in an average A260/A280 value of 1.92 +/- 0.02 and in an average A260/A230 value of 1.86 +/- 0.06.
NucleoMag technology can be easily adapted to common laboratory automation platforms
NucleoMag technology can be easily adapted to common laboratory automation platforms. It can be used either with static-pin separators (e.g., NucleoMag SEP) or magnetic separators integrated into robotic workstations.
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Robust yields and excellent performance in downstream applications
DNA was isolated from fresh 200 μL human blood samples (n=12) using the NucleoMag® Blood 200 μL kit on an epMotion® 5073m workstation. The DNA concentration of all 12 samples was determined by UV spectroscopy, dark blue bars). Performance in downstream applications was evaluated by con‑ ducting qPCR for a 250 bp sequence in the ß-actin gene. The target sequence was successfully amplified in all samples (orange squares = CT values).
Highly pure nucleic acids from human blood samples
DNA was isolated from fresh 200 μL human blood samples (n=12) using the NucleoMag Blood 200 μL kit on a epMotion® 5073m workstation. The purity was determined by UV spectroscopy. DNA quality analysis resulted in an average A260/A280 value of 1.92 +/- 0.02 and in an average A260/A230 value of 1.86 +/- 0.06.
NucleoMag technology can be easily adapted to common laboratory automation platforms
NucleoMag technology can be easily adapted to common laboratory automation platforms. It can be used either with static-pin separators (e.g., NucleoMag SEP) or magnetic separators integrated into robotic workstations.
