Applications

• Manual and automated isolation of circulating cell-free DNA from human plasma, derived from e.g., EDTA blood draw tubes or Cell-Free DNA BCT® for “Liquid Biopsy” analysis
• Typical downstream applications: NGS, qPCR, ddPCR

Features

• Consistent cfDNA recovery from 1–10 mL plasma samples
• Efficient purification of fragmented DNA as small as 50 bp
• No PCR inhibition regardless of your preferred sample volume

Specifications

• Technology: Magnetic-bead technology
• Format: Highly reactive superparamagnetic beads
• Processing: Manual or automated
• Sample material: 1–10 mL human EDTA/Cell-Free DNA BCT® plasma
• Fragment size: > 50 bp
• Typical yield: Depending on sample source, storage, and quality
• A260/A280: 1.6–1.9
• Elution volume: 50–200 µL
• Preparation time: 60 min/24 preps (2 mL; excl. lysis)*
• Binding capacity: 0.3 µg/µL beads