Applications

• Manual or automated isolation of DNA from bacteria and yeasts
• Typical downstream applications: PCR, Southern blotting, any kind of enzymatic reaction

Features

• Magnetic beads-based, automation friendly kit for DNA isolation from diverse microbial samples
• Environmentally friendly buffer chemistry free of dangerous goods
• Compatible with diverse solutions for sample homogenization

Specifications

• Technology: Magnetic bead technology
• Format: Highly reactive superparamagnetic beads
• Processing: Manual or automated
• Sample material: < 40 mg wet weight microbial cell culture pellets of Gram-positive and Gram-negative bacteria, or yeasts
• Fragment size: 300 bp–approx. 50 kbp
• Typical yield: Varies by sample and disruption device. See application data in product flyer for some typical results.
• A260/A280: 1.6–1.9
• Elution volume: 50–200 µL
• Preparation time: 30–50 min for 96 samples on KingFisher® Flex (excl. sample lysis; depending on protocol)*
• Binding capacity: 0.4 µg/µL beads

*Depending on instrument type, setup, and configuration.