NucleoBond Xtra Midi
NucleoBond Xtra Midi
Brand: MACHEREY-NAGEL
In stock
SKU
NucleoBond Xtra Midi
Transfection-grade plasmid DNA isolation. Column filter for fast lysate clarification
Applications
- High- and low‑copy* plasmid DNA purification from E. coli cultures
Features
- Column filter for fast and easy lysate clarification included – high filter flow rates, parallel lysate clearing and loading onto the column
- Midi and Maxi preps with extremely high yield
- NucleoSpin® Finishers, NucleoSnap® Finishers, and NucleoBond® Finalizers are available as separate tools to avoid time consuming centrifugation for plasmid precipitation
Specifications
- Technology: Anion exchange chromatography, gravity flow columns
- Endotoxin level: 1–10 EU/μg DNA
- Sample material: < 200 mL (high‑copy plasmid), < 400 mL (low‑copy plasmid)
- Typical yield: 400 μg
- Processing time: 70 min/prep (NucleoBond® Xtra Midi), 30 min/prep (NucleoBond® Xtra Midi Plus, incl. NucleoBond® Finalizer)
Yield in comparison to competitor anion exchange based kits
Plasmid DNA was isolated following each manufacturer’s protocol using the maximum culture volume with high plasmid content. Yield of plasmid DNA was determined after DNA precipitation. Comparison: A) Kits including desalting tool, B) Kits without desalting tool.
Shorter preparation time compared to competitors
NucleoBond® Xtra shows up to 60 % time saving and up to 100 % higher yields compared to competitor products. Comparison: A) Kits including desalting tool, B) Kits without desalting tool.
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Yield in comparison to competitor anion exchange based kits
Plasmid DNA was isolated following each manufacturer’s protocol using the maximum culture volume with high plasmid content. Yield of plasmid DNA was determined after DNA precipitation. Comparison: A) Kits including desalting tool, B) Kits without desalting tool.
Shorter preparation time compared to competitors
NucleoBond® Xtra shows up to 60 % time saving and up to 100 % higher yields compared to competitor products. Comparison: A) Kits including desalting tool, B) Kits without desalting tool.
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