NucleoBond Xtra Midi EF
NucleoBond Xtra Midi EF
Brand: MACHEREY-NAGEL
In stock
SKU
NucleoBond Xtra Midi EF
Applications
- High‑copy and low‑copy plasmid DNA purification from E. coli culture
Features
- Plasmid DNA with less than 0.05 EU/μg for transfection of highly sensitive cells (e.g., primary cells, stem cells)
- Patented endotoxin removal by additional washing step – no extra ice incubation necessary
- Column filter included in Midi / Maxi columns – high filter flow rates, parallel lysate clearing and loading onto the column ensures fast processing
Specifications
- Technology: Anion exchange chromatography, gravity flow columns
- Processing: Manual
- Endotoxin level: < 0.05 EU/μg DNA
- Plasmid type: High-, low‑copy*
- Sample material: < 200 mL (high‑copy plasmid), < 400 mL (low‑copy plasmid)
- Vector size: < 300 kbp
- Typical yield: 400 μg
- Processing time: 85 min/prep, 45 min/prep (NucleoBond® Xtra Midi Plus EF)
Efficient transfection of endotoxin-sensitive eukaryotic cells
Huh-7 cells were transfected with Lipofectamine® 2000 reagent (Life Technologies). The viability of cells treated with plasmid DNA isolated with the NucleoBond® Xtra Midi EF kit is comparable to the viability of cells in the control, indicating that the plasmid DNA does not affect cell viability.
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Efficient transfection of endotoxin-sensitive eukaryotic cells
Huh-7 cells were transfected with Lipofectamine® 2000 reagent (Life Technologies). The viability of cells treated with plasmid DNA isolated with the NucleoBond® Xtra Midi EF kit is comparable to the viability of cells in the control, indicating that the plasmid DNA does not affect cell viability.
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