NucleoBond Xtra BAC
NucleoBond Xtra BAC
Brand: MACHEREY-NAGEL
In stock
SKU
NucleoBond Xtra BAC
Applications
- Large construct (P1, BACs, PACs) plasmid purification from E. coli cultures
Features
- Optimized column design – one prep in less than 75 min
- Column filter for lysate clarification included – parallel lysate clearing and loading onto the column ensures fast processing
- Optimized silica material – yields up to 150 μg
Specifications
- Technology: Anion exchange chromatography, gravity flow columns
- Endotoxin level: 1–10 EU/μg DNA
- Sample material: 250–750 mL
- Vector size: < 300 kbp
- Typical yield: 10–150 μg
- Binding capacity: 150 μg
- Processing time: 75 min/2–4 preps
Yield in comparison to competitor anion exchange based kits
Plasmid DNA was isolated following each manufacturer’s protocol using the maximum culture volume with high plasmid content. Yield of plasmid DNA was determined after DNA precipitation. Comparison: A) Kits including desalting tool, B) Kits without desalting tool.
Shorter preparation time compared to competitors
NucleoBond® Xtra shows up to 60 % time saving and up to 100 % higher yields compared to competitor products. Comparison: A) Kits including desalting tool, B) Kits without desalting tool.
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Yield in comparison to competitor anion exchange based kits
Plasmid DNA was isolated following each manufacturer’s protocol using the maximum culture volume with high plasmid content. Yield of plasmid DNA was determined after DNA precipitation. Comparison: A) Kits including desalting tool, B) Kits without desalting tool.
Shorter preparation time compared to competitors
NucleoBond® Xtra shows up to 60 % time saving and up to 100 % higher yields compared to competitor products. Comparison: A) Kits including desalting tool, B) Kits without desalting tool.
