Applications

• RNA isolation from soil for qRT-PCR analysis
• Typical downstream applications: real-time RT-PCR

Features

• Anion exchange technology in Mini format to optimize RNA yield and purity - suitable for qRT-PCR
• Combination of mechanical homogenization and chemical lysis allows processing of large sample amounts
• Parallel preparation of RNA and DNA in one hour

Specifications

• Technology: Anion exchange chromatography, gravity flow columns combined with NucleoSpin® Bead Tubes Type A
• Format: Gravity flow columns
• Sample material: 0.25–0.5 g soil
• Fragment size: > 100 nt
• Typical yield: 0.25–2.5 μg
• A260/A280: 1.5–2.0
• Typical RIN (RNA integrity number): > 7.0
• Elution volume: 50–100 µL
• Preparation time: 60 min/12 preps
• Binding capacity: 30 µg