Applications

• High‑copy and low‑copy plasmid purification from E. coli culture

Features

• Clarification of lysates by NucleoBond® Folded Filters or Bottle Top Filters, no centrifugation required, no shearing forces
• Separate kit components available: NucleoBond® AX Columns, RNase, and buffers

Specifications

• Technology: Anion exchange chromatography, gravity flow columns
• Vector size: 300 kbp
• Endotoxin level: < 0.1 EU/μg DNA

NucleoBond® PC 500 EF

• Sample material: 30–150 mL
• Typical yield: 100–500 μg
• Processing time: 100 min/2 preps

NucleoBond® PC 2000 EF

• Sample material: 150–500 mL
• Typical yield: 500–2000 μg
• Processing time: 150 min/2 preps

NucleoBond® PC 10000 EF

• Sample material: 500–2000 mL
• Typical yield: 2000–10000 μg
• Processing time: 180 min/2 preps

NucleoBond® PC Prep 100

• Sample material: 5–20 L
• Typical yield: 80–100 mg
• Processing time: 2 h/prep