Applications

• High molecular weight DNA from mammalian tissues, blood samples, invertebrates, plants, bacteria, and yeast

Features

• High quality, high molecular weight (HMW) DNA up to 200 kbp
• Minimized DNA shearing due to established anion exchange technology
• Validated with diverse samples and sequencing platforms

Specifications

• Technology: Anion exchange chromatography
• Format: Midi gravity flow columns
• Fragment size: Up to 200 kbp with > 90% depletion of fragments < 10 kpb
• Sample material: 
• < 1.5 g plant leaves (ground under liquid nitrogen)
• < 107 cultured cells (enzymatic lysis)
• < 300 mg solid tissue (cut into small pieces and lysed enzymatically with increased lysis time, ground under liquid nitrogen, or lysed by bead beating)
• < 30 mg yeast or bacteria (ground under liquid nitrogen)
• < 300 mg yeast or bacteria (lysed enzymatically or by bead beating)
• < 2 mL liquid sample (e.g., blood, body fluids or enzymatic reactions)
• Typical yield: Depending on the sample amount and type
• Elution volume: 50–250 µL
• Processing time: 2h/12 preps (including 30 min lysis)