NucleoBond 96 Xtra EF
NucleoBond 96 Xtra EF
Brand: MACHEREY-NAGEL
In stock
SKU
NucleoBond 96 Xtra EF
Applications
- High‑copy and low‑copy plasmid DNA purification from E. coli culture
Features
- Plasmid DNA with less than 0.1 EU/μg for transfection of highly sensitive cells
- Patented endotoxin removal by additional washing step
- Processing of up to 5 mL bacterial culture
- NucleoBond® Filter Plate for convenient filtration of bacterial lysates
- Processing under vacuum or by centrifugation
- Innovative MN Wash Plate minimizes risk of cross-contamination
- NucleoBond® Finalizer Plate to speed up DNA precipitation
Specifications
- Technology: Anion exchange chromatography, gravity flow columns
- Processing: Manual or automated
- Sample material: 1–5 mL
- Plasmid type: High‑copy
- Vector size: < 15 kbp, < 300 kbp (without
- Typical yield: 2–4 μg (1.5 mL culture in 96‑well plate),10–50 μg (5 mL culture in glass tube)
- Elution volume: 100–200 μL
- Binding capacity: 50 μg
- Processing time: 120 min/96 preps
Efficient transfection of endotoxin-sensitive eukaryotic cells
Huh-7 cells were transfected with Lipofectamine® 2000 reagent (Life Technologies). The viability of cells treated with plasmid DNA isolated with the NucleoBond® Xtra Midi EF kit is comparable to the viability of cells in the control, indicating that the plasmid DNA does not affect cell viability.
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Efficient transfection of endotoxin-sensitive eukaryotic cells
Huh-7 cells were transfected with Lipofectamine® 2000 reagent (Life Technologies). The viability of cells treated with plasmid DNA isolated with the NucleoBond® Xtra Midi EF kit is comparable to the viability of cells in the control, indicating that the plasmid DNA does not affect cell viability.
