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NheI
NheI restriction site:
G | CTAG C
C GATC | G
Supplied buffer: M
Effect of DNA methylation: enzyme activity is affected by CG methylase
Reaction temperature: 37°C
- Source: Neisseria mucosa heidelbergensis
- Concentration: 4–12 units/µl
- Substrate for unit definition: lambda DNA
- Genome DNA analysis: Arthrobacter luteus genome DNA
- Star activity: unrelated site may be cut in the presence of DMSO, high concentrations of glycerol, alkaline pH, or low ionic strength
- Each lot undergoes stringent quality testing including overdigestion, genome DNA analysis, ligation-recutting and pKF3 cloning
NheI restriction site:
G | CTAG C
C GATC | G
Supplied buffer: M
Effect of DNA methylation: enzyme activity is affected by CG methylase
Reaction temperature: 37°C
- Source: Neisseria mucosa heidelbergensis
- Concentration: 4–12 units/µl
- Substrate for unit definition: lambda DNA
- Genome DNA analysis: Arthrobacter luteus genome DNA
- Star activity: unrelated site may be cut in the presence of DMSO, high concentrations of glycerol, alkaline pH, or low ionic strength
- Each lot undergoes stringent quality testing including overdigestion, genome DNA analysis, ligation-recutting and pKF3 cloning
