Neural Differentiation Medium NDiff 227
Neural Differentiation Medium NDiff 227
Brand: Takara Bio.
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Neural Differentiation Medium NDiff 227
NDiff 227 is a proprietary, defined, serum-free medium for the neural differentiation of mouse ES cells in adherent monolayer culture conditions. NDiff 227 has also been shown to support the serum-free, feeder-free culture of mouse ES cells. More recently, 227-like medium supplemented with growth factors has also been used to successfully maintain feeder-free human ES cells in culture. When supplemented with FGF, serum-free 227 also generates Anterior Definitive Endoderm (ADE) precursors from mouse ES cells, which can then be differentiated into liver and pancreas with enhanced efficiency.
Overview
- A complete, serum-free, ready‐to‐use medium for neural differentiation of mouse ES cells
- Supplemented with N2 and B-27
Applications
- Neural differentiation of mouse ES cells in monoculture
- Refer to the Data Sheet for additional examples of use
Neural differentiation of mouse embryonic stem cells cultured in adherent monoculture conditions with NDiff 227 medium
Neural differentiation of mouse embryonic stem cells cultured in adherent monoculture conditions with NDiff 227 medium.
Ying, Q.-L., Stavridis, M., Griffiths, D., Li, M. & Smith, A. Conversion of embryonic stem cells into neuroectodermal precursors in adherent monoculture. Nat. Biotechnol. 21, 183-186 (2003).
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Neural differentiation of mouse embryonic stem cells cultured in adherent monoculture conditions with NDiff 227 medium
Neural differentiation of mouse embryonic stem cells cultured in adherent monoculture conditions with NDiff 227 medium.
