Ligation-recutting test: Ligation efficiency of the cohesive ended DNA fragments generated by this enzyme is low. More efficient ligation can be achieved by using blunt end ligation reaction conditions.
Star activity: cleavage at alternative site(s) occurs in the presence of Mn2+, high concentrations of glycerol, or at high ionic strength.
Do not dilute. This enzyme is unstable at low concentration. The addition of Triton-X 100 to a final concentration of 0.01% in reaction mixture increases the relative activity up to approx. 150%.
Each lot undergoes stringent quality testing including overdigestion, genome DNA analysis, ligation-recutting and pKF3 cloning
Ligation-recutting test: Ligation efficiency of the cohesive ended DNA fragments generated by this enzyme is low. More efficient ligation can be achieved by using blunt end ligation reaction conditions.
Star activity: cleavage at alternative site(s) occurs in the presence of Mn2+, high concentrations of glycerol, or at high ionic strength.
Do not dilute. This enzyme is unstable at low concentration. The addition of Triton-X 100 to a final concentration of 0.01% in reaction mixture increases the relative activity up to approx. 150%.
Each lot undergoes stringent quality testing including overdigestion, genome DNA analysis, ligation-recutting and pKF3 cloning
