Mixture of total RNAs from a collection of adult mouse tissues, chosen to represent a broad range of expressed genes. Both male and female animals are represented. Universal Reference Total RNA serves as a standard for comparing gene expression data and is useful as an internal control for microarray expression profiling experiments.
Total RNA was isolated by a modified guanidinium thiocyanate method.
Overview
Featuring the broadest possible gene representation with minimal lot-to-lot variation
Clontech Rat Universal Reference Total RNA demonstrates more than 90% gene coverage
Clontech Rat Universal Reference Total RNA demonstrates more than 90% gene coverage. Cy3 labeled probes were generated using our Rat Reference Total RNA (Row A), another vendor’s reference total RNA (Row B), a random RNA mixture (Row C), and an individual RNA sample (Row D). Probes were hybridized to a glass rat array containing 3,766 long oligonucleotides. Expression results were analyzed using GeneSpring software (version 5.0) to cluster genes according to their expression patterns. Varying colors reflect the ratio of the intensity of any gene on each array to its median intensity across all arrays. The red and blue colors reflect high and low ratios, respectively. Our results indicate that the Reference Total RNA has more than 90% gene coverage with even distribution and outperforms another vendor’s universal RNA mixture.
Mixture of total RNAs from a collection of adult mouse tissues, chosen to represent a broad range of expressed genes. Both male and female animals are represented. Universal Reference Total RNA serves as a standard for comparing gene expression data and is useful as an internal control for microarray expression profiling experiments.
Total RNA was isolated by a modified guanidinium thiocyanate method.
Overview
Featuring the broadest possible gene representation with minimal lot-to-lot variation
Clontech Rat Universal Reference Total RNA demonstrates more than 90% gene coverage
Clontech Rat Universal Reference Total RNA demonstrates more than 90% gene coverage. Cy3 labeled probes were generated using our Rat Reference Total RNA (Row A), another vendor’s reference total RNA (Row B), a random RNA mixture (Row C), and an individual RNA sample (Row D). Probes were hybridized to a glass rat array containing 3,766 long oligonucleotides. Expression results were analyzed using GeneSpring software (version 5.0) to cluster genes according to their expression patterns. Varying colors reflect the ratio of the intensity of any gene on each array to its median intensity across all arrays. The red and blue colors reflect high and low ratios, respectively. Our results indicate that the Reference Total RNA has more than 90% gene coverage with even distribution and outperforms another vendor’s universal RNA mixture.