Mouse Spleen Marathon-Ready cDNA
Mouse Spleen Marathon-Ready cDNA
Brand: Takara Bio.
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Mouse Spleen Marathon-Ready cDNA
Marathon-Ready cDNA is high-quality, double-stranded cDNA which has been ligated to the Marathon Adaptor and is ready for use as a template in 5' and 3' Marathon RACE reactions. In many cases, the full-length cDNA can then be obtained by end-to-end amplification or standard cloning. Enough material is provided for 30 50-μl Marathon RACE reactions.
Overview
- Adaptor-ligated and ready for RACE PCR
- Tissue-specific "pool" of double-stranded cDNA
- Obtain full-length cDNAs without ever constructing or screening a cDNA library
- Perfect template for Marathon cDNA Amplification
Applications
- Rapid amplification of cDNA ends (RACE)
- Study tissue-specific gene expression
- Discover polymorphic forms of mRNA
- Obtain full-length copies of published cDNAs
- Characterization of tissue-specific patterns of gene expression
Borson, N. D., et al. A lock-docking oligo(dT) primer for 5' and 3' RACE PCR. PCR Methods Appl. 2, 144–148 (1992).
Chenchik, A., et al. Full-Length cDNA Cloning and Determination of mRNA 5' and 3' Ends by Amplification of Adaptor-Ligated cDNA. BioTechniques 21, 526–532 (1996).
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