Mouse Feeder Cell Quantification Kit
Mouse Feeder Cell Quantification Kit
Brand: Takara Bio.
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Mouse Feeder Cell Quantification Kit
The Mouse Feeder Cell Quantification Kit is designed to facilitate highly sensitive detection and quantification of genomic DNA derived from residual mouse feeder cells using real-time PCR to analyze genomic DNA extracted from stem cell cultures (e.g., hESC, hiPSC). The kit includes primers for real-time PCR that were designed to detect a mouse mitochondrial (mt) DNA sequence, allowing detection of mouse feeder cells with high sensitivity. The copy number of cellular mtDNA is known to vary depending on cell type, cell line, and differentiation state, and there is a possibility of fluctuation betweenmouse feeder cell lots. The primer set for quantification of mouse mtDNA copy number included in this kit can be used for lot-to-lot correction of mouse feeder cells.
Overview
- Utilizes real-time PCR to detect and quantify residual mouse feeder cells
- Provides mtDNA PCR primers to make mouse mtDNA sequence detection easy and sensitive
- Includes nuclear DNA PCR primers for normalization to quantify mouse mtDNA copy number
Applications
- Assess the level of mouse cell contamination in stem cell cultures grown using mouse feeder cells
- Compare the levels of mouse cell contamination in stem cell cultures grown with different lots of mouse feeder cells
Components
| TB Green Premix Ex Taq II (Tli RNaseH Plus) (2X Conc.) | 625 μl x 4 |
| Mouse_Mt_primer (10 μM each) | 100 μl |
| Mouse_genome_primer (10 μM each) | 100 μl |
| Human_primer_Type I (CHR3) (10 μM each) | 100 μl |
| Human_primer_Type II (CHR7) (10 μM each) | 100 μl |
| Human_primer_Type III (CHR15) (10 μM each) | 100 μl |
| dH2O | 1 ml x 2 |
| EASY Dilution (for Real Time PCR) | 1 ml x 2 |
| ROX Reference Dye (50X Conc.) | 50 μl |
| ROX Reference Dye II (50X Conc.) | 50 μl |
| Human Genomic DNA (100 ng/μl) | 20 μl |
Storage
- –20°C (all components except Premix Ex Taq II (Tli RNaseH Plus))
- –80°C (Premix Ex Taq II (Tli RNaseH Plus))
- Product is stable for up to 6 months when stored at 4°C. Protect from light and avoid contamination. This kit is shipped in a dry ice-packed container. For long-term storage, keep item at –80°C.
- Do not store at –20°C. Store thawed product at 4°C and use within 6 months.
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