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MluI
MluI restriction site:
A | CGCG T
T GCGC | A
Supplied buffer: H
Effect of DNA methylation: enzyme activity is affected by CG methylase
Reaction temperature: 37°C
- Source: Micrococcus luteus
- Concentration: 4–12 units/µl [High concentration: 30–60 units/µl]
- Substrate for unit definition: lambda DNA
- Genome DNA analysis: Staphylococcus aureus genome DNA
- Each lot undergoes stringent quality testing including overdigestion, genome DNA analysis, ligation-recutting and pKF3 cloning
MluI restriction site:
A | CGCG T
T GCGC | A
Supplied buffer: H
Effect of DNA methylation: enzyme activity is affected by CG methylase
Reaction temperature: 37°C
- Source: Micrococcus luteus
- Concentration: 4–12 units/µl [High concentration: 30–60 units/µl]
- Substrate for unit definition: lambda DNA
- Genome DNA analysis: Staphylococcus aureus genome DNA
- Each lot undergoes stringent quality testing including overdigestion, genome DNA analysis, ligation-recutting and pKF3 cloning
