Mir-X miRNA First-Strand Synthesis Kit
Mir-X miRNA First-Strand Synthesis Kit
Brand: Takara Bio.
In stock
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Mir-X miRNA First-Strand Synthesis Kit
Kit contains reagents for synthesizing first-strand cDNA from either total RNA or purified small RNAs isolated from any source. RNA molecules are polyadenylated and reverse transcribed using the mRQ Enzyme Mix, which contains poly(A) polymerase and SMART MMLV RT. The resulting cDNA and Takara Bio's TB Green Advantage qPCR Premix can be used in a real-time qPCR analysis to quantify specific miRNAs and other RNAs present in the original sample. For 60 rxn kit see Cat. # 638315.
Overview
- Simple, single-step cDNA synthesis reaction
- Two kits in one: cDNA synthesis and qPCR of microRNA
- Quantify any microRNA and its target using the same RNA sample
- Detection of microRNA down to 50 copies
- High specificity
- 200 rxn and 600 rxn qPCR kit formats
Applications
- cDNA synthesis from microRNA
- microRNA quantitation by qPCR
Mir-X miRNA qRT-PCR TB Green Kits use a single-step, single-tube reaction to produce first-strand cDNA, which is then specifically and quantitatively amplified using a miRNA-specific primer and TB Green Advantage qPCR chemistry
Mir-X miRNA qRT-PCR TB Green Kits use a single-step, single-tube reaction to produce first-strand cDNA, which is then specifically and quantitatively amplified using a miRNA-specific primer and TB Green Advantage qPCR chemistry. In the Mir-X cDNA synthesis reaction, RNAs are poly(A)-tailed using poly(A) polymerase, and then copied using a modified oligo(dT) primer and SMART MMLV Reverse Transcriptase.
Specific quantification of Let7 miRNA variants
Specific quantification of Let7 miRNA variants. Using miRNA-specific primers (Panel A), Mir-X qRT-PCR was able to specifically detect and quantify each member of a series of 8 synthetic Let7 variants that had been spiked into a background of yeast poly(A)+ RNA (Panel B).The primers detected each of their corresponding Let7 miRNA cognates, but did not detect the off-target variants in 63 of 64 possible combinations.
Induction and quantitation of miR-9 miRNA in mouse P19 cells
Induction and quantitation of miR-9 miRNA in mouse P19 cells. Exposing aggregated mouse P19 cell clusters to retinoic acid (RA) causes them to acquire neural cell phenotypes, which are accompanied by changes in the cellular miRNA pool. Using the Mir-X miRNA quantitation system, we tracked the abundance of one such miRNA, miR-9, which was induced by RA and continued to accumulate in these cells following a 5 day exposure to RA.
Trichostatin A treatment alters miRNA expression in mouse ES cells
Trichostatin A treatment alters miRNA expression in mouse ES cells. In mouse embryonic stem cells, we were able to monitor the alterations in expression for a panel of 12 miRNAs that respond to trichostatin A (TSA) treatment.
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Mir-X miRNA qRT-PCR TB Green Kits use a single-step, single-tube reaction to produce first-strand cDNA, which is then specifically and quantitatively amplified using a miRNA-specific primer and TB Green Advantage qPCR chemistry
Mir-X miRNA qRT-PCR TB Green Kits use a single-step, single-tube reaction to produce first-strand cDNA, which is then specifically and quantitatively amplified using a miRNA-specific primer and TB Green Advantage qPCR chemistry. In the Mir-X cDNA synthesis reaction, RNAs are poly(A)-tailed using poly(A) polymerase, and then copied using a modified oligo(dT) primer and SMART MMLV Reverse Transcriptase.
Specific quantification of Let7 miRNA variants
Specific quantification of Let7 miRNA variants. Using miRNA-specific primers (Panel A), Mir-X qRT-PCR was able to specifically detect and quantify each member of a series of 8 synthetic Let7 variants that had been spiked into a background of yeast poly(A)+ RNA (Panel B).The primers detected each of their corresponding Let7 miRNA cognates, but did not detect the off-target variants in 63 of 64 possible combinations.
Induction and quantitation of miR-9 miRNA in mouse P19 cells
Induction and quantitation of miR-9 miRNA in mouse P19 cells. Exposing aggregated mouse P19 cell clusters to retinoic acid (RA) causes them to acquire neural cell phenotypes, which are accompanied by changes in the cellular miRNA pool. Using the Mir-X miRNA quantitation system, we tracked the abundance of one such miRNA, miR-9, which was induced by RA and continued to accumulate in these cells following a 5 day exposure to RA.
Trichostatin A treatment alters miRNA expression in mouse ES cells
Trichostatin A treatment alters miRNA expression in mouse ES cells. In mouse embryonic stem cells, we were able to monitor the alterations in expression for a panel of 12 miRNAs that respond to trichostatin A (TSA) treatment.
